Characterization of interstitial Cajal progenitors cells and their changes in Hirschsprung's disease

• Published in: PloS one 2014-01, Vol.9 (1), p.e86100-e86100
• Main Authors: Chen, Zhi-Hua, Zhang, Yong-Chang, Jiang, Wei-Fang, Yang, Cissy, Zou, Gang-Ming, Kong, Yu, Cai, Wei
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biology; Bone marrow; c-Kit protein; Case-Control Studies; Caveolae; CD34 antigen; Cells, Cultured; Child, Preschool; Colon; Confocal; Confocal microscopy; Congenital diseases; Constipation; Cristae; Cytometry; Degranulation; Diabetes; Drug dosages; Electron microscopy; Endoplasmic reticulum; Enteric nervous system; Female; Flow cytometry; Gastric motility; Gastroenterology; Genotype & phenotype; Hirschsprung Disease - metabolism; Hospitals; Humans; Immunophenotyping; Infant; Interstitial cells; Interstitial cells of Cajal; Interstitial Cells of Cajal - cytology; Interstitial Cells of Cajal - metabolism; Interstitial Cells of Cajal - ultrastructure; Localization; Male; Medicine; Middle Aged; Mitochondria; Motility; Muscles; Mutation; Myoblasts, Smooth Muscle - cytology; Myoblasts, Smooth Muscle - metabolism; Myoblasts, Smooth Muscle - ultrastructure; Nerves; Neurosciences; Pathogenesis; Pediatrics; Phenotype; Rodents; Small intestine; Smooth muscle; Stem cells; Studies; Surgery; Transmission electron microscopy; Tumors; Ultrastructure
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0086100

Interstitial cells of Cajal (ICC) are critical to gastrointestinal motility. The phenotypes of ICC progenitors have been observed in the mouse gut, but whether they exist in the human colon and what abnormal changes in their quantity and ultrastructure are present in Hirschsprung's disease (HSCR) colon remains uncertain. In this study, we collected the surgical resection of colons, both proximal and narrow segments, from HSCR patients and normal controls. First, we identified the progenitor of ICC in normal adult colon using immunofluorescent localization techniques with laser confocal microscopy. Next, the progenitors were sorted to observe their morphology. We further applied flow cytometry to examine the content of ICC progenitors in these fresh samples. The ultrastructural changes in the narrow and proximal parts of the HSCR colon were observed using transmission electron microscopy (TEM) and were compared with the normal adult colon. The presumed early progenitor (c-Kit(low)CD34(+)Igf1r(+)) and committed progenitor (c-Kit(+)CD34(+)Igf1r(+)) of ICC exist in adult normal colon as well as in the narrow and proximal parts of the HSCR colon. However, the proportions of mature, early and committed progenitors of ICC were dramatically reduced in the narrow segment of the HSCR colon. The proportions of mature and committed progenitors of ICC in the proximal segment of the HSCR colon were lower than in the adult normal colon. Ultrastructurally, ICC, enteric nerves, and smooth muscle in the narrow segment of the HSCR colon showed severe injury, including swollen vacuola or ted mitochondria, disappearance of mitochondrial cristae, dilated rough endoplasmic reticulum, vesiculation and degranulation, and disappearance of the caveolae on the ICC membrane surface. The contents of ICC and its progenitors in the narrow part of the HSCR colon were significantly decreased than those of adult colon, which may be associated with HSCR pathogenesis.