Influence of the presence of B chromosomes on DNA damage in Crepis capillaris

The sensitivity of different plant species to mutagenic agents is related to the DNA content and organization of the chromatin, which have been described in ABCW and bodyguard hypotheses, respectively. Plant species that have B chromosomes are good models for the study of these hypotheses. This stud...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e87337
Main Authors: Kwasniewska, Jolanta, Mikolajczyk, Agnieszka
Format: Article
Language:English
Subjects:
Allium
Bioassays
Biology
Chromatin
Chromosomes
Chromosomes, Plant - genetics
Comet Assay
Correlation analysis
Crepis - genetics
Crepis capillaris
Damage detection
Deoxyribonucleic acid
DNA
DNA damage
DNA Damage - drug effects
DNA Damage - genetics
DNA methylation
Euchromatin
Flowers & plants
Gene sequencing
Heterochromatin
Hypotheses
Impact damage
In Situ Hybridization, Fluorescence
Maleic hydrazide
Maleic Hydrazide - toxicity
Mutagens
Mutation
Nucleotide sequence
RNA, Ribosomal - genetics
Toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The sensitivity of different plant species to mutagenic agents is related to the DNA content and organization of the chromatin, which have been described in ABCW and bodyguard hypotheses, respectively. Plant species that have B chromosomes are good models for the study of these hypotheses. This study presents an analysis of the correlation between the occurrence of B chromosomes and the DNA damage that is induced by the chemical mutagen, maleic hydrazide (MH), in Crepis capillaris plants using comet assay. The presence of B chromosomes has a detectable impact on the level of DNA damage. The level of DNA damage after MH treatment was correlated with the number of B chromosomes and it was observed that it increased significantly in plants with 3B chromosomes. We did not find evidence of the protective role from chemical mutagens of the constitutive heterochromatin for euchromatin in relation to DNA damage. The DNA damage involving the 25S rDNA sequences was analyzed using the comet-FISH technique. Fragmentation within or near the 25S rDNA involved the loci on the A and B chromosomes. The presence of B chromosomes in C. capillaris cells had an influence on the level of DNA damage that involves the 25S rDNA region.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087337