Pronounced inflammatory response to endotoxaemia during nighttime: a randomised cross-over trial

• Published in: PloS one 2014-01, Vol.9 (1), p.e87413-e87413
• Main Authors: Alamili, Mahdi, Bendtzen, Klaus, Lykkesfeldt, Jens, Rosenberg, Jacob, Gögenur, Ismail
• Format: Article
• Language: English
• Subjects: Acids; Adipokines - blood; Alcohol; Analysis; Analysis of Variance; Ascorbic acid; Biology; Blood pressure; Caffeine; Chitinase-3-Like Protein 1; Circadian rhythm; Circadian Rhythm - physiology; Circadian rhythms; Cross-Over Studies; Cytokines; Cytokines - blood; E coli; Endotoxemia - physiopathology; Escherichia coli; Free radicals; Gastroenterology; Gene expression; Heart rate; Hospitals; Human behavior; Humans; Infection; Infections; Inflammation; Inflammation - physiopathology; Inflammatory response; Interleukin 1; Interleukin 1 receptor antagonist; Interleukin 1 Receptor Antagonist Protein - blood; Interleukin 10; Interleukin 6; Interleukin-10 - blood; Interleukin-1beta - blood; Interleukin-6 - blood; Interleukins; Lectins - blood; Lipopolysaccharides; Lipopolysaccharides - administration & dosage; Male; Malondialdehyde; Malondialdehyde - blood; Medicine; Melatonin; Menstruation; Mitogens; Night; Organic acids; Oxidative stress; Oxidative Stress - physiology; Receptors; Receptors, Tumor Necrosis Factor - blood; Rodents; Sepsis; Sleep; Stress response; Studies; Surgery; Time Factors; TNF inhibitors; Tumor necrosis factor; Tumor necrosis factor receptors; Tumor Necrosis Factor-alpha - blood; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0087413

Circadian variation in bodily functions has been shown to impact health in acute and chronic medical conditions. Little is known about the relationship between circadian rhythm and sepsis in humans. We aimed to investigate circadian variations in the host response in a human endotoxaemia model. A cross-over study, where 12 healthy young men received E. coli endotoxin (lipopolysaccharide, LPS) 0.3 ng/kg at 12 noon and, on another day, at 12 midnight. Blood samples were analysed for pro- and anti-inflammatory cytokines: tumour-necrosis factor (TNF)-alpha, soluble TNF receptors (sTNF-R)-1 and -2, interleukin (IL)-1beta, IL-1 receptor antagonist (IL-1Ra), IL-6, and IL-10 as well as YKL-40 and the oxidative stress markers malondialdehyde (MDA), ascorbic acid (AA) and dehydroascorbic acid (DHA) before and at 2, 4, 6 and 8 hours after LPS administration. The levels of MDA and IL-10 where significantly higher during the day time (P