Progesterone treatment shows benefit in a pediatric model of moderate to severe bilateral brain injury

Controlled cortical impact (CCI) models in adult and aged Sprague-Dawley (SD) rats have been used extensively to study medial prefrontal cortex (mPFC) injury and the effects of post-injury progesterone treatment, but the hormone's effects after traumatic brain injury (TBI) in juvenile animals h...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e87252-e87252
Main Authors: Geddes, Rastafa I, Sribnick, Eric A, Sayeed, Iqbal, Stein, Donald G
Format: Article
Language:English
Subjects:
Age
Age Factors
Analysis
Animal models
Animals
Behavior, Animal - drug effects
Biology
Body weight
Brain
Brain injuries
Brain Injuries - drug therapy
Brain Injuries - pathology
Cortex (frontal)
Drug dosages
Edema
Emergency medical care
Females
Fuel consumption
Head injuries
Male
Males
Medical research
Medicine
Neuroprotection
Pediatrics
Prefrontal cortex
Progesterone
Progesterone - administration & dosage
Progesterone - therapeutic use
Progestins - administration & dosage
Progestins - therapeutic use
Rats
Rats, Sprague-Dawley
Rodents
Traumatic brain injury
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Controlled cortical impact (CCI) models in adult and aged Sprague-Dawley (SD) rats have been used extensively to study medial prefrontal cortex (mPFC) injury and the effects of post-injury progesterone treatment, but the hormone's effects after traumatic brain injury (TBI) in juvenile animals have not been determined. In the present proof-of-concept study we investigated whether progesterone had neuroprotective effects in a pediatric model of moderate to severe bilateral brain injury. Twenty-eight-day old (PND 28) male Sprague Dawley rats received sham (n = 24) or CCI (n = 47) injury and were given progesterone (4, 8, or 16 mg/kg per 100 g body weight) or vehicle injections on post-injury days (PID) 1-7, subjected to behavioral testing from PID 9-27, and analyzed for lesion size at PID 28. The 8 and 16 mg/kg doses of progesterone were observed to be most beneficial in reducing the effect of CCI on lesion size and behavior in PND 28 male SD rats. Our findings suggest that a midline CCI injury to the frontal cortex will reliably produce a moderate TBI comparable to what is seen in the adult male rat and that progesterone can ameliorate the injury-induced deficits.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087252