Urine microRNA as potential biomarkers of autosomal dominant polycystic kidney disease progression: description of miRNA profiles at baseline

Autosomal dominant polycystic kidney disease (ADPKD) is clinically heterogenic. Biomarkers are needed to predict prognosis and guide management. We aimed to profile microRNA (miRNA) in ADPKD to gain molecular insight and evaluate biomarker potential. Small-RNA libraries were generated from urine spe...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e86856-e86856
Main Authors: Ben-Dov, Iddo Z, Tan, Ying-Cai, Morozov, Pavel, Wilson, Patricia D, Rennert, Hanna, Blumenfeld, Jon D, Tuschl, Thomas
Format: Article
Language:English
Subjects:
Adult
Aged
Analysis
Biological markers
Biology
Biomarkers
Biomarkers - urine
Cancer
Cell culture
Chronic kidney failure
Cistrons
Cysts
Development and progression
Disease Progression
Epigenesis, Genetic
Epithelial cells
Etiology
Female
Fetus
Fetuses
Gene expression
Gene Library
High-Throughput Nucleotide Sequencing
Hospitals
Humans
Inflammation
Kidney diseases
Kidney transplantation
Kidney Tubules - metabolism
Kidney Tubules - pathology
Laboratories
Male
Medical research
Medicine
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - urine
Middle Aged
miRNA
Molecular biology
Nephrology
Pathogenesis
Pathology
Patients
Polycystic kidney
Polycystic kidney disease
Polycystic Kidney, Autosomal Dominant - diagnosis
Polycystic Kidney, Autosomal Dominant - genetics
Polycystic Kidney, Autosomal Dominant - pathology
Polycystic Kidney, Autosomal Dominant - urine
Primary Cell Culture
Prognosis
Proteins
Regulation
Ribonucleic acid
RNA
RNA polymerase
Suppressors
Urine
Urothelium - metabolism
Urothelium - pathology
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Description
Summary:Autosomal dominant polycystic kidney disease (ADPKD) is clinically heterogenic. Biomarkers are needed to predict prognosis and guide management. We aimed to profile microRNA (miRNA) in ADPKD to gain molecular insight and evaluate biomarker potential. Small-RNA libraries were generated from urine specimens of ADPKD patients (N = 20) and patients with chronic kidney disease of other etiologies (CKD, N = 20). In this report, we describe the miRNA profiles and baseline characteristics. For reference, we also examined the miRNA transcriptome in primary cultures of ADPKD cyst epithelia (N = 10), normal adult tubule (N = 8) and fetal tubule (N = 7) epithelia. In primary cultures of ADPKD kidney cells, miRNA cistrons mir-143(2) (9.2-fold), let-7i(1) (2.3-fold) and mir-3619(1) (12.1-fold) were significantly elevated compared to normal tubule epithelia, whereas mir-1(4) members (19.7-fold), mir-133b(2) (21.1-fold) and mir-205(1) (3.0-fold) were downregulated (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0086856