Decreased expression of the GATA3 gene is associated with poor prognosis in primary gastric adenocarcinoma

GATA binding protein 3 (GATA3) was recently proposed to function as a tumor suppressor gene in some types of human cancer. This study aims to investigate GATA3 expression and its prognostic significance in primary gastric adenocarcinoma. Using real-time quantitative PCR (RT-qPCR) and immunohistochem...

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Bibliographic Details
Published in:PloS one 2014-02, Vol.9 (2), p.e87195-e87195
Main Authors: Keshari, Rajiv Prasad, Wang, Wei, Zhang, Yu, Wang, Dan-dan, Li, Yuan-fang, Yuan, Shu-qiang, Qiu, Hai-bo, Huang, Chun-yu, Chen, Yong-ming, Xia, Jian-chuan, Zhou, Zhi-wei
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Adenocarcinoma - surgery
Aged
Breast cancer
Cancer genetics
Cancer research
Cancer therapies
Collaboration
Deoxyribonucleic acid
Development and progression
DNA
Female
Gastrectomy
Gastric cancer
GATA-3 protein
GATA3 Transcription Factor - genetics
GATA3 Transcription Factor - metabolism
Gene expression
Gene Expression Regulation, Neoplastic
Genes
Genes, Neoplasm - genetics
Genetic aspects
Genetic research
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Laboratories
Male
Mathematical models
Medical prognosis
Medicine
Middle Aged
Morphogenesis
Multivariate Analysis
Oncology
Ovarian cancer
Patients
Prenatal development
Prognosis
Protein binding
Proteins
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Rodents
Stomach cancer
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach Neoplasms - surgery
Surgery
Survival
Transcription factors
Tumor suppressor genes
Tumors
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Items that cite this one
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Summary:GATA binding protein 3 (GATA3) was recently proposed to function as a tumor suppressor gene in some types of human cancer. This study aims to investigate GATA3 expression and its prognostic significance in primary gastric adenocarcinoma. Using real-time quantitative PCR (RT-qPCR) and immunohistochemical staining methods, GATA3 expression was analyzed in tissue samples from a consecutive series of 402 gastric adenocarcinoma patients who underwent resections between 2003 and 2006. The relationship between GATA3 expression, clinicopathological factors, and patient survival was investigated. The expression status of GATA3 was shown to be clearly reduced in the tumor tissue samples compared with that in the matched adjacent non-tumor tissue samples by RT-qPCR (P = 0.0014). Immunohistochemistry analysis indicated that GATA3 expression was significantly decreased in 225 of the 402 (56%) gastric adenocarcinoma cases. Reduced GATA3 expression was also observed in patients with large tumors (P = 0.017), signet ring cell carcinoma or mucinous carcinoma (P = 0.005) and tumors with lymphatic or venous invasion (P = 0.040). Additionally, reduced expression of GATA3 was more commonly observed in tumors that were staged as T4a/b (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087195