MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha

Aberrant expression of miR-196a has been frequently reported in different cancers including pancreatic cancer. However, its function in pancreatic cancer has not been fully elucidated. Here, we investigated the expression pattern and the biological role of miR-196a in pancreatic cancer cell lines, a...

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Published in:PloS one 2014-02, Vol.9 (2), p.e87897-e87897
Main Authors: Huang, Fengting, Tang, Jian, Zhuang, Xiaohong, Zhuang, Yanyan, Cheng, Wenjie, Chen, Wenbo, Yao, Herui, Zhang, Shineng
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Aberration
Apoptosis
Apoptosis - genetics
Base Sequence
Biology
Biotechnology
Cancer
Cancer metastasis
Cell Cycle - genetics
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation
Cluster Analysis
Colorectal cancer
Comparative analysis
Cyclin D1
Cyclin-dependent kinase 4
Disease Progression
DNA microarrays
E-cadherin
Epithelial cells
Gastroenterology
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Silencing
Growth factors
Humans
I-kappa B Proteins - chemistry
I-kappa B Proteins - genetics
Inhibitors
Luciferase
Lymphoma
Medical prognosis
Medicine
Melanoma
Metastases
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - chemistry
MicroRNAs - genetics
miRNA
N-Cadherin
NF-KappaB Inhibitor alpha
Pancreatic cancer
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Penicillin
RNA Interference
Skin cancer
Target recognition
Tumor cell lines
Vimentin
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container_title PloS one
container_volume 9
creator Huang, Fengting
Tang, Jian
Zhuang, Xiaohong
Zhuang, Yanyan
Cheng, Wenjie
Chen, Wenbo
Yao, Herui
Zhang, Shineng
description Aberrant expression of miR-196a has been frequently reported in different cancers including pancreatic cancer. However, its function in pancreatic cancer has not been fully elucidated. Here, we investigated the expression pattern and the biological role of miR-196a in pancreatic cancer cell lines, as well as its interaction with a metastasis-related gene, nuclear factor-kappa-B-inhibitor alpha (NFKBIA). We demonstrated that miR-196a was up-regulated in human pancreatic cancer cell lines compared with immortalized pancreatic ductal epithelial cells by means of microRNAs microarray and qRT-PCR. Furthermore, down-regulation of miR-196a in PANC-1 suppressed its proliferation and migration with an increase in G0/G1 transition and decreased expression of Cyclin D1 and CDK4/6. Meanwhile, an increased expression in E-cadherin and decreased expression in N-cadherin and Vimentin were also observed. We identified a novel miR-196a target, NFKBIA, and down-regulation of miR-196a enhanced the expression of NFKBIA protein. Luciferase assay confirmed that NFKBIA was a direct and specific target of miR-196a. Silencing NFKBIA in PANC-1 cells enhanced its proliferation and migration. Taken together, our findings indicate that miR-196a is highly expressed in pancreatic cancer cell lines, and may play a crucial role in pancreatic cancer proliferation and migration, possibly through its downstream target, NFKBIA. Thus, miR-196a may serve as a potential therapeutic target for pancreatic cancer.
doi_str_mv 10.1371/journal.pone.0087897
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genetics</topic><topic>Base Sequence</topic><topic>Biology</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cancer metastasis</topic><topic>Cell Cycle - genetics</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell Proliferation</topic><topic>Cluster Analysis</topic><topic>Colorectal cancer</topic><topic>Comparative analysis</topic><topic>Cyclin D1</topic><topic>Cyclin-dependent kinase 4</topic><topic>Disease Progression</topic><topic>DNA microarrays</topic><topic>E-cadherin</topic><topic>Epithelial cells</topic><topic>Gastroenterology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Silencing</topic><topic>Growth factors</topic><topic>Humans</topic><topic>I-kappa B Proteins - chemistry</topic><topic>I-kappa B Proteins - genetics</topic><topic>Inhibitors</topic><topic>Luciferase</topic><topic>Lymphoma</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>Melanoma</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - chemistry</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>N-Cadherin</topic><topic>NF-KappaB Inhibitor alpha</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Penicillin</topic><topic>RNA Interference</topic><topic>Skin cancer</topic><topic>Target recognition</topic><topic>Tumor cell lines</topic><topic>Vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Fengting</creatorcontrib><creatorcontrib>Tang, Jian</creatorcontrib><creatorcontrib>Zhuang, Xiaohong</creatorcontrib><creatorcontrib>Zhuang, Yanyan</creatorcontrib><creatorcontrib>Cheng, Wenjie</creatorcontrib><creatorcontrib>Chen, Wenbo</creatorcontrib><creatorcontrib>Yao, Herui</creatorcontrib><creatorcontrib>Zhang, Shineng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - 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However, its function in pancreatic cancer has not been fully elucidated. Here, we investigated the expression pattern and the biological role of miR-196a in pancreatic cancer cell lines, as well as its interaction with a metastasis-related gene, nuclear factor-kappa-B-inhibitor alpha (NFKBIA). We demonstrated that miR-196a was up-regulated in human pancreatic cancer cell lines compared with immortalized pancreatic ductal epithelial cells by means of microRNAs microarray and qRT-PCR. Furthermore, down-regulation of miR-196a in PANC-1 suppressed its proliferation and migration with an increase in G0/G1 transition and decreased expression of Cyclin D1 and CDK4/6. Meanwhile, an increased expression in E-cadherin and decreased expression in N-cadherin and Vimentin were also observed. We identified a novel miR-196a target, NFKBIA, and down-regulation of miR-196a enhanced the expression of NFKBIA protein. Luciferase assay confirmed that NFKBIA was a direct and specific target of miR-196a. Silencing NFKBIA in PANC-1 cells enhanced its proliferation and migration. Taken together, our findings indicate that miR-196a is highly expressed in pancreatic cancer cell lines, and may play a crucial role in pancreatic cancer proliferation and migration, possibly through its downstream target, NFKBIA. Thus, miR-196a may serve as a potential therapeutic target for pancreatic cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24504166</pmid><doi>10.1371/journal.pone.0087897</doi><tpages>e87897</tpages><oa>free_for_read</oa></addata></record>
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subjects 3' Untranslated Regions
Aberration
Apoptosis
Apoptosis - genetics
Base Sequence
Biology
Biotechnology
Cancer
Cancer metastasis
Cell Cycle - genetics
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation
Cluster Analysis
Colorectal cancer
Comparative analysis
Cyclin D1
Cyclin-dependent kinase 4
Disease Progression
DNA microarrays
E-cadherin
Epithelial cells
Gastroenterology
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Silencing
Growth factors
Humans
I-kappa B Proteins - chemistry
I-kappa B Proteins - genetics
Inhibitors
Luciferase
Lymphoma
Medical prognosis
Medicine
Melanoma
Metastases
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - chemistry
MicroRNAs - genetics
miRNA
N-Cadherin
NF-KappaB Inhibitor alpha
Pancreatic cancer
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Penicillin
RNA Interference
Skin cancer
Target recognition
Tumor cell lines
Vimentin
title MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha
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