Whole genome analysis of a community-associated methicillin-resistant Staphylococcus aureus ST59 isolate from a case of human sepsis and severe pneumonia in China

• Published in: PloS one 2014-02, Vol.9 (2), p.e89235-e89235
• Main Authors: Qu, Tingting, Feng, Ye, Jiang, Yan, Zhu, Peiqiong, Wei, Zeqing, Chen, Yan, Otto, Michael, Yu, Yunsong
• Format: Article
• Language: English
• Subjects: Adolescent; Anti-Bacterial Agents - administration & dosage; Antibiotics; Antimicrobial agents; Beta lactamases; Biology; Case reports; Cephalosporins; China; Cloning; Communities; Community-Acquired Infections - drug therapy; Community-Acquired Infections - genetics; Community-Acquired Infections - microbiology; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Drug resistance; Genes; Genetic testing; Genome, Bacterial - genetics; Genomes; Genomics; Hospitals; Humans; Immunoglobulins, Intravenous - administration & dosage; Infectious diseases; Intravenous administration; Laboratories; Leukocidin; Male; Medical diagnosis; Medical schools; Medicine; Methicillin; Methicillin-Resistant Staphylococcus aureus - drug effects; Methicillin-Resistant Staphylococcus aureus - genetics; Methicillin-Resistant Staphylococcus aureus - isolation & purification; Microbial drug resistance; Microbial Sensitivity Tests; Multilocus Sequence Typing; Phylogeny; Pneumonia; Pneumonia, Staphylococcal - drug therapy; Pneumonia, Staphylococcal - genetics; Pneumonia, Staphylococcal - microbiology; Sepsis; Sepsis - drug therapy; Sepsis - genetics; Sepsis - microbiology; Sequence Analysis, DNA; Skin; Staphylococcal Infections - drug therapy; Staphylococcal Infections - genetics; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus infections
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089235

We report a case of necrotizing pneumonia in a young patient caused by community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) in a teaching hospital in the People's Republic of China. The patient had a typical clinical presentation and was successfully treated with antibiotics and intravenous immunoglobulin. A CA-MRSA strain, named SA268, was isolated from the blood of the patient. The isolate was susceptible to most antimicrobial agents, except cephalosporins, penicillins, and β-lactamase inhibitor combinations. Multi-locus sequence typing (MLST) assigned SA268 to ST59, a clone widely spread in eastern Asia. The strain was positive for Panton Valentine Leukocidin (PVL)-encoding genes and SCCmec type V. We sequenced the complete genome of the SA268 isolate. The genome of SA268 was almost identical to that of the Taiwanese ST59 CA-MRSA strains M013 and SA957. However, we observed several differences in gene composition, which included differences in the SCCmec element and several lipoprotein genes that were present in the Taiwanese strains but absent from SA268.