Analysis of the intestinal lumen microbiota in an animal model of colorectal cancer

Recent reports have suggested that multiple factors such as host genetics, environment and diet can promote the progression of healthy mucosa towards sporadic colorectal carcinoma. Accumulating evidence has additionally associated intestinal bacteria with disease initiation and progression. In order...

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Bibliographic Details
Published in:PloS one 2014-03, Vol.9 (6), p.e90849
Main Authors: Zhu, Qingchao, Jin, Zhiming, Wu, Wen, Gao, Renyuan, Guo, Bomin, Gao, Zhiguang, Yang, Yongzhi, Qin, Huanlong
Format: Article
Language:English
Subjects:
Abundance
Analysis
Animal models
Animals
Bacteria
Bacterial infections
Bacteroides
Bacteroides - genetics
Biodiversity
Cancer
Colon
Colon - microbiology
Colon cancer
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - chemically induced
Colorectal Neoplasms - microbiology
Digestive system
Digestive tract
Flora
Fusobacterium
Fusobacterium nucleatum
Gastroenterology
Gastrointestinal tract
Genes, Bacterial
Genetics
Infections
Inflammatory bowel disease
Intestinal microflora
Intestine
Laboratory animals
Lactobacillus
Male
Medicine
Microbiota
Microbiota (Symbiotic organisms)
Microbiota - genetics
Microorganisms
Molecular Typing
Mucosa
Phylogenetics
Prevotella - genetics
Principal components analysis
Probiotics
Proteobacteria - genetics
Rats
Rats, Wistar
RNA
Rodents
rRNA 16S
Sequence Analysis, DNA
Species
Species diversity
Spirochetes
Studies
Surgery
Treponema - genetics
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Summary:Recent reports have suggested that multiple factors such as host genetics, environment and diet can promote the progression of healthy mucosa towards sporadic colorectal carcinoma. Accumulating evidence has additionally associated intestinal bacteria with disease initiation and progression. In order to examine and analyze the composition of gut microbiota in the absence of confounding influences, we have established an animal model of 1, 2-dimethylhydrazine (DMH)-induced colon cancer. Using this model, we have performed pyrosequencing of the V3 region of the 16S rRNA genes in this study to determine the diversity and breadth of the intestinal microbial species. Our findings indicate that the microbial composition of the intestinal lumen differs significantly between control and tumor groups. The abundance of Firmicutes was elevated whereas the abundance of Bacteroidetes and Spirochetes was reduced in the lumen of CRC rats. Fusobacteria was not detected in any of the healthy rats and there was no significant difference in observed Proteobacteria species when comparing the bacterial communities between our two groups. Interestingly, the abundance of Proteobacteria was higher in CRC rats. At the genus level, Bacteroides exhibited a relatively higher abundance in CRC rats compared to controls (14.92% vs. 9.22%, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0090849