Smooth muscle enfoldment internal sphincter construction after intersphincteric resection for rectal cancer

• Published in: PloS one 2014-03, Vol.9 (3), p.e91491-e91491
• Main Authors: Jin, Heiying, Zhang, Bei, Yao, Hang, Du, Yonghong, Wang, Xiaofeng, Leng, Qiang
• Format: Article
• Language: English
• Subjects: Anal Canal - surgery; Animals; Anus; Biology; Cancer; Chinese medicine; Clinical trials; Colon; Colorectal cancer; Colorectal surgery; Construction; Digestive System Surgical Procedures; Disease Models, Animal; Fecal incontinence; Fecal Incontinence - surgery; Female; Hogs; Male; Manometry; Medical research; Medicine; Muscle, Smooth - pathology; Muscles; Pigs; Pressure; Quality of life; Reconstructive Surgical Procedures; Rectal Neoplasms - physiopathology; Rectal Neoplasms - surgery; Rectum; Smooth muscle; Sphincter; Surgery; Swine; Swine, Miniature; Systematic review; Ultrasound
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0091491

To assess smooth muscle enfoldment and internal sphincter construction (SMESC) for improvement of continence after intersphincteric resection (ISR) for rectal cancer. Twenty-four Bama miniature pigs were randomly divided into a conventional ISR group and experimental SMESC group, with 12 pigs in each group. The proximal sigmoid colon was anastomosed directly to the anus in the ISR group. In the SMESC group, internal sphincter construction was performed. At 12 weeks before and after surgery, rectal resting pressure and anal canal length were assessed. Three-dimensional ultrasound was used to determine the thickness of the internal sphincter. After the animals were sacrificed, the rectum and anus were resected and pathological examinations were performed to evaluate the differences in sphincter thickness and muscle fibers. All 24 animals in the SMESC group and the ISR group survived the surgery. Twelve weeks post-surgery, the rectal resting pressure, length of the anal high-pressure zone and the postoperative internal sphincter thickness for the ISR group were significantly lower than for the SMESC group. There was a thickened area (about 2 cm) above the anastomotic stoma among animals from the SMESC group; in addition, the smooth muscles were significantly enlarged and enfolded when compared to the ISR group. This animal model study shows that the SMESC procedure achieved acceptable reconstruction of the internal anal neo-sphincter (IAN/S), without increasing surgical risk. However, the findings in this experimental animal model must be confirmed by clinical trials to determine the safety and efficacy of this procedure in clinical practice.