Monocyte expression and soluble levels of the haemoglobin receptor (CD163/sCD163) and the mannose receptor (MR/sMR) in septic and critically ill non-septic ICU patients

• Published in: PloS one 2014-03, Vol.9 (3), p.e92331-e92331
• Main Authors: Kjærgaard, Anders G, Rødgaard-Hansen, Sidsel, Dige, Anders, Krog, Jan, Møller, Holger J, Tønnesen, Else
• Format: Article
• Language: English
• Subjects: Aged; Anesthesiology; Antigens, CD - blood; Antigens, CD - metabolism; Antigens, Differentiation, Myelomonocytic - blood; Antigens, Differentiation, Myelomonocytic - metabolism; Biochemistry; Biology and Life Sciences; Biomarkers; CD163 antigen; Cell activation; Cell Survival; Critical Illness; Cytometry; Demography; Diagnostic systems; Enzyme-linked immunosorbent assay; Female; Flow cytometry; Haptoglobin; Hemoglobin; Hospitalization; Hospitals; Humans; Illnesses; Intensive care; Intensive Care Units; Lectins, C-Type - blood; Lectins, C-Type - metabolism; Leukocyte Count; Macrophages; Male; Mannose; Mannose-Binding Lectins - blood; Mannose-Binding Lectins - metabolism; Medical diagnosis; Medical prognosis; Medicine and Health Sciences; Middle Aged; Monocytes; Monocytes - metabolism; Monocytes - pathology; Patients; Protein Binding; Receptors; Receptors, Cell Surface - blood; Receptors, Cell Surface - metabolism; Research and Analysis Methods; ROC Curve; Sepsis; Sepsis - blood; Sepsis - metabolism; Septic shock; Solubility; Studies
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0092331

The diagnosis of sepsis is challenging and there is an unmet need for sensitive and specific diagnostic and prognostic biomarkers. Following activation of macrophages and monocytes, the haptoglobin-haemoglobin receptor (CD163) and the mannose receptor (MR) are shed into the circulation (sCD163 and sMR). We investigated monocyte expression of CD163 and MR, and levels of sCD163 and sMR in septic and non-septic patients, and in healthy controls. We hypothesised that these receptors are elevated during sepsis and can be used diagnostic and prognostic. Twenty-one patients with severe sepsis or septic shock and 15 critically ill non-septic patients were included in this prospective observational study at three ICUs at Aarhus University Hospital and Randers Regional Hospital, Denmark. Fifteen age- and gender-matched healthy volunteers served as controls. Levels of sCD163 and sMR were measured using a sandwich ELISA and monocyte expression of CD163 and MR was evaluated by flow cytometry during the first four days of ICU stay. The diagnostic and prognostic values of the receptors were assessed using AUROC curves. At ICU admission and during the observation period, monocyte expression of CD163 and levels of sCD163 and sMR were significantly higher in septic patients compared with non-septic patients and healthy controls (p