Toxicity and biodistribution of the serotype 2 recombinant adeno-associated viral vector, encoding Aquaporin-1, after retroductal delivery to a single mouse parotid gland

• Published in: PloS one 2014-03, Vol.9 (3), p.e92832
• Main Authors: Momot, Dariya, Zheng, Changyu, Yin, Hongen, Elbekai, Reem H, Vallant, Molly, Chiorini, John A
• Format: Article
• Language: English
• Subjects: Animal rights; Animals; Antibodies; Aquaporin 1; Aquaporins; Biology and Life Sciences; Blood; Body weight; Cannulation; Clinical trials; Coding; Dependovirus; Exocrine glands; Females; Food; Food consumption; Gene therapy; Genetic Vectors - adverse effects; Genetic Vectors - genetics; Genetic Vectors - pharmacology; Head & neck cancer; Heart diseases; Hematocrit; Hematology; Humans; Indicators; Kidneys; Leukocytes; Leukocytes (neutrophilic); Liver; Lymph nodes; Males; Medical research; Medicine and Health Sciences; Mice; Mice, Inbred BALB C; Parotid gland; Parotid Gland - metabolism; Parotid Gland - pathology; Pathology; Physiology; Proteins; Quality of life; Radiation; Radiation damage; Radiation effects; Research and Analysis Methods; Rodents; Safety; Salivary gland; Studies; Toxicity; Toxicology; Transduction, Genetic - methods; Vectors (Biology); Water consumption
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0092832

In preparation for testing the safety of using serotype 2 recombinant adeno-associated vector, encoding Aquaporin-1 to treat radiation-induced salivary gland damage in a phase 1 clinical trial, we conducted a 13 week GLP biodistribution and toxicology study using Balb/c mice. To best assess the safety of rAAV2hAQP1 as well as resemble clinical delivery, vector (10(8), 10(9), 10(10), or 4.4 × 10(10) vector particles/gland) or saline was delivered to the right parotid gland of mice via retroductal cannulation. Very mild surgically induced inflammation was caused by this procedure, seen in 3.6% of animals for the right parotid gland, and 5.3% for the left parotid gland. Long term distribution of vector appeared to be localized to the site of cannulation as well as the right and left draining submandibular lymph nodes at levels >50 copies/μg in some animals. As expected, there was a dose-related increase in neutralizing antibodies produced by day 29. Overall, animals appeared to thrive, with no differences in mean body weight, food or water consumption between groups. There were no significant adverse effects due to treatment noted by clinical chemistry and pathology evaluations. Hematology assessment of serum demonstrated very limited changes to the white blood cell, segmented neutrophils, and hematocrit levels and were concluded to not be vector-associated. Indicators for liver, kidney, cardiac functions and general tissue damage showed no changes due to treatment. All of these indicators suggest the treatment is clinically safe.