Metabolomic analysis of diet-induced type 2 diabetes using UPLC/MS integrated with pattern recognition approach

Metabolomics represents an emerging discipline concerned with comprehensive assessment of small molecule endogenous metabolites in biological systems and provides a powerful approach insight into the mechanisms of diseases. Type 2 diabetes (T2D), called the burden of the 21st century, is growing wit...

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Bibliographic Details
Published in:PloS one 2014-03, Vol.9 (3), p.e93384-e93384
Main Authors: Sun, Hui, Zhang, Shuxiang, Zhang, Aihua, Yan, Guangli, Wu, Xiuhong, Han, Ying, Wang, Xijun
Format: Article
Language:English
Subjects:
Acetaldehyde
Animals
Biology and Life Sciences
Biomarkers
Biomarkers - urine
Chromatography
Development and progression
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 2 - etiology
Diabetes Mellitus, Type 2 - urine
Diet
Diet therapy
Diet, High-Fat - adverse effects
Discriminant analysis
Disease
Epidemics
Homeostasis
Laboratory animals
Male
Mass spectrometry
Medicine
Medicine and Health Sciences
Metabolic disorders
Metabolic pathways
Metabolism
Metabolites
Metabolome
Metabolomics
Obesity
Pathogenesis
Pattern recognition
Pattern Recognition, Automated
Pentose
Pharmaceuticals
Physical Sciences
Rats, Wistar
Research and Analysis Methods
Rodents
Scientific imaging
Starch
Sucrose
Sugar
Sulfates
Tandem Mass Spectrometry
Type 2 diabetes
Tyrosine
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Summary:Metabolomics represents an emerging discipline concerned with comprehensive assessment of small molecule endogenous metabolites in biological systems and provides a powerful approach insight into the mechanisms of diseases. Type 2 diabetes (T2D), called the burden of the 21st century, is growing with an epidemic rate. However, its precise molecular mechanism has not been comprehensively explored. In this study, we applied urinary metabolomics based on the UPLC/MS integrated with pattern recognition approaches to discover differentiating metabolites, to characterize and explore metabolic pathway disruption in an experimental model for high-fat-diet induced T2D. Six differentiating urinary metabolites were found in the negative mode, and two (2-(4-hydroxy-3-methoxy-phenyl) acetaldehyde sulfate, 2-phenylethanol glucuronide) of which were identified involving the key metabolic pathways linked to pentose and glucuronate interconversions, starch, sucrose metabolism and tyrosine metabolism. Our study provides new insight into pathophysiologic mechanisms and may enhance the understanding of T2D pathogenesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0093384