Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource

• Published in: PloS one 2014-03, Vol.9 (3), p.e93275-e93275
• Main Authors: Xu, Xiao, Lv, Qiming, Shang, Junjun, Pang, Zhiqian, Zhou, Zhuangzhi, Wang, Jing, Jiang, Guanghuai, Tao, Yong, Xu, Qian, Li, Xiaobing, Zhao, Xianfeng, Li, Shigui, Xu, Jichen, Zhu, Lihuang
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Sequence; Amino acids; Analysis; Binding sites; Biology and Life Sciences; Breeding; Breeding - methods; China; Chromosomes; Cloning; Developmental biology; Disease; Disease resistance; Disease Resistance - genetics; Engineering; Excavation; Genes; Genes, Plant - genetics; Genetic engineering; Genetically modified plants; Genetics; Genomes; Genomics; Inoculation; Laboratories; Magnaporthe - genetics; Magnaporthe oryzae; Mining; NBS-LRR protein; Oryza; Oryza - genetics; Oryza rufipogon; Oryza sativa indica; Oryza sativa japonica; Phylogeny; Plant breeding; Plant diseases; Plant Diseases - genetics; Plant genetics; Plant immunity; Plant Proteins - genetics; Plants, Genetically Modified - genetics; Proteins; Rice; Rice blast; Science; Sequence Analysis, DNA; Sequences; Transgenic plants
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0093275

Twenty-six orthologs of the rice blast resistance gene Pid3 from cultivated varieties and wild rice accessions distributed in different areas were cloned by allele mining. Sequence analysis showed that while each of the orthologous genes from indica varieties and most wild accessions encodes a complete NBS-LRR protein, each of the proteins encoded by those from japonica varieties and few wild rice accessions presents a premature termination. Eleven of the 26 orthologs were selected for blast resistance testing by transforming into the blast susceptible rice variety TP309, respectively. Inoculation of 23 M. oryzae strains collected from diverse regions of China to the respective transgenic plants revealed that 6 Pid3 orthologs showed susceptible to all the tested strains, while the other 5 orthologs showed differential resistance spectra in a gradually spectrum-widen order as Pid3-W3, Pid3-W4, Pid3-I3, Pid3-W5 and Pid3-I1. Amino acid sequences alignment of these orthologs indicated that the sequence diversities between the blast resistance orthologs were mostly located in the LRR domain such as the substitutions of Q694H,D856H,Q896R,D899E etc. However, the differences between the resistance orthologs and the susceptible ones were mostly located in the NBS domain. The present experiments provide an example of that the ortholog evaluation of plant R genes could be an efficient way to expand the rice blast resistance and some other plant disease resistance as well for breeding.