Start-up of an anaerobic dynamic membrane digester for waste activated sludge digestion: temporal variations in microbial communities

An anaerobic dynamic membrane digester (ADMD) was developed to digest waste sludge, and pyrosequencing was used to analyze the variations of the bacterial and archaeal communities during the start-up. Results showed that bacterial community richness decreased and then increased over time, while bact...

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Bibliographic Details
Published in:PloS one 2014-04, Vol.9 (4), p.e93710
Main Authors: Yu, Hongguang, Wang, Qiaoying, Wang, Zhiwei, Sahinkaya, Erkan, Li, Yongli, Ma, Jinxing, Wu, Zhichao
Format: Article
Language:English
Subjects:
Activated sludge
Anaerobic microorganisms
Analysis
Bacteria
Biological diversity
Biology and Life Sciences
Bioreactors - microbiology
Cloning
Communities
Consortia
Cytophaga
Digesters
Engineering
Engineering and Technology
Environmental science
Laboratories
Membrane reactors
Methane - metabolism
Methane production
Methanogenesis
Methanomicrobiales - isolation & purification
Methanosarcinales - isolation & purification
Microbial activity
Microorganisms
Phylogenetics
Proteobacteria - isolation & purification
Sewage - microbiology
Sludge
Sludge digestion
Studies
Temporal variations
Water treatment
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Summary:An anaerobic dynamic membrane digester (ADMD) was developed to digest waste sludge, and pyrosequencing was used to analyze the variations of the bacterial and archaeal communities during the start-up. Results showed that bacterial community richness decreased and then increased over time, while bacterial diversity remained almost the same during the start-up. Proteobacteria and Bacteroidetes were the major phyla. At the class level, Betaproteobacteria was the most abundant at the end of start-up, followed by Sphingobacteria. In the archaeal community, richness and diversity peaked at the end of the start-up stage. Principle component and cluster analyses demonstrated that archaeal consortia experienced a distinct shift and became stable after day 38. Methanomicrobiales and Methanosarcinales were the two predominant orders. Further investigations indicated that Methanolinea and Methanosaeta were responsible for methane production in the ADMD system. Hydrogenotrophic pathways might prevail over acetoclastic means for methanogenesis during the start-up, supported by specific methanogenic activity tests.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0093710