Evidence of circadian rhythm, oxygen regulation capacity, metabolic repeatability and positive correlations between forced and spontaneous maximal metabolic rates in lake sturgeon Acipenser fulvescens

• Published in: PloS one 2014-04, Vol.9 (4), p.e94693-e94693
• Main Authors: Svendsen, Jon C, Genz, Janet, Anderson, W Gary, Stol, Jennifer A, Watkinson, Douglas A, Enders, Eva C
• Format: Article
• Language: English
• Subjects: Acipenser fulvescens; Acipenser medirostris; Acipenser naccarii; Acipenseridae; Animal behavior; Animals; Basal Metabolism; Behavior, Animal; Biology and Life Sciences; Body mass; Body Weight; Circadian rhythm; Circadian Rhythm - physiology; Circadian rhythms; Correlation; Environmental regulations; Environmental science; Environmental stress; Fish; Fisheries; Fishes - metabolism; Fishing; Genetic aspects; Hypoxia; Laboratories; Lakes; Metabolic rate; Metabolism; Natural selection; Oreochromis niloticus; Oxygen; Oxygen - metabolism; Photoperiod; Physical fitness; Physiological aspects; Physiological transport; Physiology; Regulation; Reproducibility; Reproducibility of Results; Respiration; Respirometry; Rhythm; Stress response; Sturgeon; Sturgeons; Time Factors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0094693

Animal metabolic rate is variable and may be affected by endogenous and exogenous factors, but such relationships remain poorly understood in many primitive fishes, including members of the family Acipenseridae (sturgeons). Using juvenile lake sturgeon (Acipenser fulvescens), the objective of this study was to test four hypotheses: 1) A. fulvescens exhibits a circadian rhythm influencing metabolic rate and behaviour; 2) A. fulvescens has the capacity to regulate metabolic rate when exposed to environmental hypoxia; 3) measurements of forced maximum metabolic rate (MMR(F)) are repeatable in individual fish; and 4) MMR(F) correlates positively with spontaneous maximum metabolic rate (MMR(S)). Metabolic rates were measured using intermittent flow respirometry, and a standard chase protocol was employed to elicit MMR(F). Trials lasting 24 h were used to measure standard metabolic rate (SMR) and MMR(S). Repeatability and correlations between MMR(F) and MMR(S) were analyzed using residual body mass corrected values. Results revealed that A. fulvescens exhibit a circadian rhythm in metabolic rate, with metabolism peaking at dawn. SMR was unaffected by hypoxia (30% air saturation (O(2sat))), demonstrating oxygen regulation. In contrast, MMR(F) was affected by hypoxia and decreased across the range from 100% O(2sat) to 70% O(2sat). MMR(F) was repeatable in individual fish, and MMR(F) correlated positively with MMR(S), but the relationships between MMR(F) and MMR(S) were only revealed in fish exposed to hypoxia or 24 h constant light (i.e. environmental stressor). Our study provides evidence that the physiology of A. fulvescens is influenced by a circadian rhythm and suggests that A. fulvescens is an oxygen regulator, like most teleost fish. Finally, metabolic repeatability and positive correlations between MMR(F) and MMR(S) support the conjecture that MMR(F) represents a measure of organism performance that could be a target of natural selection.