Redefining the PF06864 Pfam Family Based on Burkholderia pseudomallei PilO2Bp S-SAD Crystal Structure

Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2Bp, a protein...

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Bibliographic Details
Published in:PloS one 2014-04, Vol.9 (4), p.e94981
Main Authors: Lassaux, Patricia, Conchillo-Solé, Oscar, Manjasetty, Babu A., Yero, Daniel, Perletti, Lucia, Belrhali, Hassan, Daura, Xavier, Gourlay, Louise J., Bolognesi, Martino
Format: Article
Language:English
Subjects:
Actin
Adenosine triphosphatase
Amino acids
Assembly
Bacteria
Bioinformatics
Biology and Life Sciences
Biotechnology
Burkholderia pseudomallei
Cloning
Crystal structure
E coli
Escherichia coli
Filaments
Genes
Genomes
Gram-negative bacteria
Laboratories
Machinery
Machinery and equipment
Markov chains
Molecular biology
Nanostructured materials
Physical Sciences
Pili
Salmonella
Salmonella Typhimurium
Three dimensional models
Vibrio cholerae
Virulence
Virulence factors
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Summary:Type IV pili are surface-exposed filaments and bacterial virulence factors, represented by the Tfpa and Tfpb types, which assemble via specific machineries. The Tfpb group is further divided into seven variants, linked to heterogeneity in the assembly machineries. Here we focus on PilO2Bp, a protein component of the Tfpb R64 thin pilus variant assembly machinery from the pathogen Burkholderia pseudomallei. PilO2Bp belongs to the PF06864 Pfam family, for which an improved definition is presented based on newly derived Hidden Markov Model (HMM) profiles. The 3D structure of the N-terminal domain of PilO2Bp (N-PilO2Bp), here reported, is the first structural representative of the PF06864 family. N-PilO2Bp presents an actin-like ATPase fold that is shown to be present in BfpC, a different variant assembly protein; the new HMM profiles classify BfpC as a PF06864 member. Our results provide structural insight into the PF06864 family and on the Type IV pili assembly machinery.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0094981