Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening

Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, meta...

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Published in:PloS one 2014-04, Vol.9 (4), p.e95041-e95041
Main Authors: San Martin-Uriz, Patxi, Mirete, Salvador, Alcolea, Pedro J, Gomez, Manuel J, Amils, Ricardo, Gonzalez-Pastor, Jose E
Format: Article
Language:English
Subjects:
Acidiphilium - genetics
Acidiphilium - metabolism
Amino acids
Arsenic
ATP-Dependent Proteases - genetics
Bacteria - classification
Bacteria - genetics
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biology and Life Sciences
Biosynthesis
Cell division
Chemical synthesis
Dehydrogenases
Drug Resistance, Bacterial - genetics
E coli
Ecology and Environmental Sciences
Enzymes
Escherichia coli
Gene expression
Gene Transfer, Horizontal
Genes
Genome, Bacterial - genetics
Genomes
Genomic Library
Lipopolysaccharides
Lipopolysaccharides - biosynthesis
Medical screening
Metals
Microbial Viability - drug effects
Microbial Viability - genetics
Microorganisms
Molecular modelling
Molecular Sequence Data
Nickel
Nickel - metabolism
Nickel - pharmacology
Open Reading Frames - genetics
Operon
Phylogeny
Protease
Proteinase
Proteins
Rivers
Rivers - microbiology
Sequence Analysis, DNA
Spain
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cited_by cdi_FETCH-LOGICAL-c596t-4d27825d029e29128e64dc9e9d036a8d02bbc24ae1de95a191adfa2aae077f3f3
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Mirete, Salvador
Alcolea, Pedro J
Gomez, Manuel J
Amils, Ricardo
Gonzalez-Pastor, Jose E
description Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance revealed a remarkable ability to tolerate high Ni concentrations. Here we report the screening of a genomic library of Acidiphilium sp. PM to identify genes involved in Ni resistance. This approach revealed seven different genes conferring Ni resistance to E. coli, two of which form an operon encoding the ATP-dependent protease HslVU (ClpQY). This protease was found to enhance resistance to both Ni and Co in E. coli, a function not previously reported. Other Ni-resistance determinants include genes involved in lipopolysaccharide biosynthesis and the synthesis of branched amino acids. The diversity of molecular functions of the genes recovered in the screening suggests that Ni resistance in Acidiphilium sp. PM probably relies on different molecular mechanisms.
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subjects Acidiphilium - genetics
Acidiphilium - metabolism
Amino acids
Arsenic
ATP-Dependent Proteases - genetics
Bacteria - classification
Bacteria - genetics
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biology and Life Sciences
Biosynthesis
Cell division
Chemical synthesis
Dehydrogenases
Drug Resistance, Bacterial - genetics
E coli
Ecology and Environmental Sciences
Enzymes
Escherichia coli
Gene expression
Gene Transfer, Horizontal
Genes
Genome, Bacterial - genetics
Genomes
Genomic Library
Lipopolysaccharides
Lipopolysaccharides - biosynthesis
Medical screening
Metals
Microbial Viability - drug effects
Microbial Viability - genetics
Microorganisms
Molecular modelling
Molecular Sequence Data
Nickel
Nickel - metabolism
Nickel - pharmacology
Open Reading Frames - genetics
Operon
Phylogeny
Protease
Proteinase
Proteins
Rivers
Rivers - microbiology
Sequence Analysis, DNA
Spain
title Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening
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