Mucosal adjuvant activity of IL-2 presenting spores of bacillus subtilis in a murine model of Helicobacter pylori vaccination

The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant...

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Bibliographic Details
Published in:PloS one 2014-04, Vol.9 (4), p.e95187-e95187
Main Authors: Hinc, Krzysztof, Stasiłojć, Małgorzata, Piątek, Iwona, Peszyńska-Sularz, Grażyna, Isticato, Rachele, Ricca, Ezio, Obuchowski, Michał, Iwanicki, Adam
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Animal models
Animals
Antigens
Bacillus
Bacillus subtilis
Bacillus subtilis - physiology
Bacteria
Bacterial vaccines
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Bacterial Vaccines - microbiology
Biology and Life Sciences
Biotechnology
Chromosomes
Cloning
Deoxyribonucleic acid
DNA
E coli
Environmental conditions
Escherichia coli
Female
Health aspects
Helicobacter Infections - genetics
Helicobacter Infections - immunology
Helicobacter Infections - metabolism
Helicobacter Infections - prevention & control
Helicobacter pylori
Helicobacter pylori - immunology
High resistance
Immune response
Immune response (cell-mediated)
Immune system
Immunization
Infections
Interleukin 2
Interleukin-2 - biosynthesis
Interleukin-2 - genetics
Interleukin-2 - immunology
Mice
Mice, Inbred BALB C
Mucosa
Physiological aspects
Proteins
Research and Analysis Methods
Spores
Spores, Bacterial - genetics
Spores, Bacterial - immunology
Spores, Bacterial - metabolism
UreB gene
Vaccination
Vaccines
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Summary:The endospores of Bacillus subtilis are now widely used as a platform for presentation of heterologous proteins and due to their safety record and high resistance to harsh environmental conditions can be considered as potential vehicles for oral vaccination. In this research we show that recombinant B. subtilis spores presenting a fragment of the Helicobacter acinonychis UreB protein and expressing the ureB gene under vegetative promoter elicit a strong cellular immune response in orally immunized mice when co-administered with spores presenting IL-2. We show for the first time the successful application of two types of recombinant spores, one carrying an antigen and the other an adjuvant, in a single oral immunization.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0095187