Sphingosine 1-phosphate (S1P) induced interleukin-8 (IL-8) release is mediated by S1P receptor 2 and nuclear factor κB in BEAS-2B cells

The airway epithelium may release pro-inflammatory cytokines and chemokines in the asthmatic airway. Sphingosine 1-phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial ce...

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Published in:PloS one 2014-04, Vol.9 (4), p.e95566-e95566
Main Authors: O'Sullivan, Michael J, Hirota, Nobuaki, Martin, James G
Format: Article
Language:English
Subjects:
Asthma
Biology and Life Sciences
Cell Line
Chemokines
Cytokines
Downstream effects
Epidermal growth factor
Epidermal growth factor receptors
Epithelial cells
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Epithelium
G protein-coupled receptors
Humans
Inflammation
Interleukin
Interleukin 8
Interleukin-8 - metabolism
Kinases
Laboratories
Leukocytes (neutrophilic)
Lysophospholipids - pharmacology
Medicine and Health Sciences
Neutrophils
NF-kappa B - metabolism
NF-κB protein
Oxidative stress
Oxygen
Pathogenesis
Penicillin
Phosphates
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Receptor, Epidermal Growth Factor - metabolism
Receptors
Receptors, Lysosphingolipid - metabolism
Respiratory tract diseases
Rodents
Signaling
Smooth muscle
Sphingosine - analogs & derivatives
Sphingosine - pharmacology
Sphingosine 1-phosphate
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description The airway epithelium may release pro-inflammatory cytokines and chemokines in the asthmatic airway. Sphingosine 1-phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial cells. S1P is a ligand for 5 G protein-coupled receptors, S1PR1-5. We wished to explore the mechanisms of S1P induced IL-8 secretion with regard to the receptor(s) and downstream signaling events involved. Our results indicate that S1P induced IL-8 release is mediated by S1PR2 and the transcription factor NF-κB. Since the Epidermal Growth Factor Receptor (EGFR) and reactive oxygen species (ROS) have been implicated in IL-8 release in response to activation of other G protein-coupled receptors, we examined their importance in S1P induced IL-8 release and established that they are not involved. This study reveals S1PR2 and NF-κB as potential therapeutic targets in neutrophilic airway diseases such as severe asthma.
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Sphingosine 1-phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial cells. S1P is a ligand for 5 G protein-coupled receptors, S1PR1-5. We wished to explore the mechanisms of S1P induced IL-8 secretion with regard to the receptor(s) and downstream signaling events involved. Our results indicate that S1P induced IL-8 release is mediated by S1PR2 and the transcription factor NF-κB. Since the Epidermal Growth Factor Receptor (EGFR) and reactive oxygen species (ROS) have been implicated in IL-8 release in response to activation of other G protein-coupled receptors, we examined their importance in S1P induced IL-8 release and established that they are not involved. 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Sphingosine 1-phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial cells. S1P is a ligand for 5 G protein-coupled receptors, S1PR1-5. We wished to explore the mechanisms of S1P induced IL-8 secretion with regard to the receptor(s) and downstream signaling events involved. Our results indicate that S1P induced IL-8 release is mediated by S1PR2 and the transcription factor NF-κB. Since the Epidermal Growth Factor Receptor (EGFR) and reactive oxygen species (ROS) have been implicated in IL-8 release in response to activation of other G protein-coupled receptors, we examined their importance in S1P induced IL-8 release and established that they are not involved. 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subjects Asthma
Biology and Life Sciences
Cell Line
Chemokines
Cytokines
Downstream effects
Epidermal growth factor
Epidermal growth factor receptors
Epithelial cells
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Epithelium
G protein-coupled receptors
Humans
Inflammation
Interleukin
Interleukin 8
Interleukin-8 - metabolism
Kinases
Laboratories
Leukocytes (neutrophilic)
Lysophospholipids - pharmacology
Medicine and Health Sciences
Neutrophils
NF-kappa B - metabolism
NF-κB protein
Oxidative stress
Oxygen
Pathogenesis
Penicillin
Phosphates
Proteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Receptor, Epidermal Growth Factor - metabolism
Receptors
Receptors, Lysosphingolipid - metabolism
Respiratory tract diseases
Rodents
Signaling
Smooth muscle
Sphingosine - analogs & derivatives
Sphingosine - pharmacology
Sphingosine 1-phosphate
title Sphingosine 1-phosphate (S1P) induced interleukin-8 (IL-8) release is mediated by S1P receptor 2 and nuclear factor κB in BEAS-2B cells
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