Immunosuppressive activity of daphnetin, one of coumarin derivatives, is mediated through suppression of NF-κB and NFAT signaling pathways in mouse T cells

Daphnetin, a plant-derived dihydroxylated derivative of coumarin, is an effective compound extracted from a plant called Daphne Korean Nakai. Coumarin derivates were known for their antithrombotic, anti-inflammatory, and antioxidant activities. The present study was aimed to determine the immunosupp...

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Published in:PloS one 2014-05, Vol.9 (5), p.e96502
Main Authors: Song, Bocui, Wang, Zhenning, Liu, Yan, Xu, Sisi, Huang, Guoren, Xiong, Ying, Zhang, Shuang, Xu, Linli, Deng, Xuming, Guan, Shuang
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Autoimmune diseases
Biology and Life Sciences
Cell cycle
Cell Proliferation - drug effects
Concanavalin A
Concanavalin A - immunology
Coumarin
Coumarins - immunology
Coumarins - pharmacology
Cytokines
Dinitrofluorobenzene
Down-Regulation - drug effects
Down-Regulation - immunology
Education
Food
Food quality
G1 Phase - drug effects
G1 Phase - immunology
Hypersensitivity
Hypersensitivity (delayed)
Hypersensitivity, Delayed - immunology
Immunoglobulins
Immunology
Immunosuppressive agents
Immunosuppressive Agents - immunology
Immunosuppressive Agents - pharmacology
Inflammation
Kinases
Laboratories
Light
Lymphocytes
Lymphocytes T
Male
Medicine and Health Sciences
Mice
Mice, Inbred BALB C
NF-AT protein
NF-kappa B - immunology
NF-κB protein
NFATC Transcription Factors - immunology
Pathways
Physical Sciences
Plant cells
Plant extracts
Plants
Proteins
Research and Analysis Methods
Resting Phase, Cell Cycle - drug effects
Resting Phase, Cell Cycle - immunology
Signal transduction
Signal Transduction - drug effects
Signal Transduction - immunology
Signaling
Studies
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
Umbelliferones - immunology
Umbelliferones - pharmacology
Veterinary colleges
Veterinary medicine
Zoonoses
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Liu, Yan
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Guan, Shuang
description Daphnetin, a plant-derived dihydroxylated derivative of coumarin, is an effective compound extracted from a plant called Daphne Korean Nakai. Coumarin derivates were known for their antithrombotic, anti-inflammatory, and antioxidant activities. The present study was aimed to determine the immunosuppressive effects and the underlying mechanisms of daphnetin on concanavalin A (ConA) induced T lymphocytes in mice. We showed that, in vitro, daphnetin suppressed ConA-induced splenocyte proliferation, influenced production of the cytokines and inhibited cell cycle progression through the G0/G1 transition. The data also revealed that daphnetin could down-regulate activation of ConA induced NF-κB and NFAT signal transduction pathways in mouse T lymphocyte. In vivo, daphnetin treatment significantly inhibited the 2, 4- dinitrofluorobenzene (DNFB) -induced delayed type hypersensitivity (DTH) reactions in mice. Collectively, daphnetin had strong immunosuppressive activity both in vitro and in vivo, suggesting a potential role for daphnetin as an immunosuppressive agent, and established the groundwork for further research on daphnetin.
doi_str_mv 10.1371/journal.pone.0096502
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Coumarin derivates were known for their antithrombotic, anti-inflammatory, and antioxidant activities. The present study was aimed to determine the immunosuppressive effects and the underlying mechanisms of daphnetin on concanavalin A (ConA) induced T lymphocytes in mice. We showed that, in vitro, daphnetin suppressed ConA-induced splenocyte proliferation, influenced production of the cytokines and inhibited cell cycle progression through the G0/G1 transition. The data also revealed that daphnetin could down-regulate activation of ConA induced NF-κB and NFAT signal transduction pathways in mouse T lymphocyte. In vivo, daphnetin treatment significantly inhibited the 2, 4- dinitrofluorobenzene (DNFB) -induced delayed type hypersensitivity (DTH) reactions in mice. Collectively, daphnetin had strong immunosuppressive activity both in vitro and in vivo, suggesting a potential role for daphnetin as an immunosuppressive agent, and established the groundwork for further research on daphnetin.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24800925</pmid><doi>10.1371/journal.pone.0096502</doi><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2014-05, Vol.9 (5), p.e96502
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1521422644
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central Free
subjects Animals
Antioxidants
Autoimmune diseases
Biology and Life Sciences
Cell cycle
Cell Proliferation - drug effects
Concanavalin A
Concanavalin A - immunology
Coumarin
Coumarins - immunology
Coumarins - pharmacology
Cytokines
Dinitrofluorobenzene
Down-Regulation - drug effects
Down-Regulation - immunology
Education
Food
Food quality
G1 Phase - drug effects
G1 Phase - immunology
Hypersensitivity
Hypersensitivity (delayed)
Hypersensitivity, Delayed - immunology
Immunoglobulins
Immunology
Immunosuppressive agents
Immunosuppressive Agents - immunology
Immunosuppressive Agents - pharmacology
Inflammation
Kinases
Laboratories
Light
Lymphocytes
Lymphocytes T
Male
Medicine and Health Sciences
Mice
Mice, Inbred BALB C
NF-AT protein
NF-kappa B - immunology
NF-κB protein
NFATC Transcription Factors - immunology
Pathways
Physical Sciences
Plant cells
Plant extracts
Plants
Proteins
Research and Analysis Methods
Resting Phase, Cell Cycle - drug effects
Resting Phase, Cell Cycle - immunology
Signal transduction
Signal Transduction - drug effects
Signal Transduction - immunology
Signaling
Studies
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
Umbelliferones - immunology
Umbelliferones - pharmacology
Veterinary colleges
Veterinary medicine
Zoonoses
title Immunosuppressive activity of daphnetin, one of coumarin derivatives, is mediated through suppression of NF-κB and NFAT signaling pathways in mouse T cells
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