The sonic hedgehog signaling pathway induces myopic development by activating matrix metalloproteinase (MMP)-2 in Guinea pigs

To investigate whether the Sonic hedgehog (Shh) signaling induces myopic development by increasing the expression of matrix metalloproteinase (MMP)-2 in guinea pigs. A translucent diffuser was glued onto the right eye to induce form-deprivation myopia (FDM) in 10 guinea pigs. Four guinea pigs were s...

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Bibliographic Details
Published in:PloS one 2014-05, Vol.9 (5), p.e96952
Main Authors: Chen, Minjie, Qian, Yishan, Dai, Jinhui, Chu, Renyuan
Format: Article
Language:English
Subjects:
Animals
Bovine serum albumin
Deprivation
Diffusers
Down-Regulation
Elongation
Enzyme Activation - drug effects
Eye
Gastric cancer
Gelatinase A
Guinea Pigs
Hedgehog protein
Hedgehog Proteins - metabolism
Kinases
Laboratories
Liver cancer
Matrix metalloproteinase
Matrix Metalloproteinase 2 - metabolism
Medicine and Health Sciences
Metalloproteinase
Myopia
Myopia - metabolism
Myopia - pathology
Myopia - physiopathology
Refraction
Refraction, Ocular - drug effects
Retina - drug effects
Retina - pathology
Retina - physiopathology
Rodents
Sclera - drug effects
Sclera - pathology
Sclera - physiopathology
Serum albumin
Signal transduction
Signal Transduction - drug effects
Signaling
Stomach cancer
Studies
Veratrum Alkaloids - pharmacology
Western blotting
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Summary:To investigate whether the Sonic hedgehog (Shh) signaling induces myopic development by increasing the expression of matrix metalloproteinase (MMP)-2 in guinea pigs. A translucent diffuser was glued onto the right eye to induce form-deprivation myopia (FDM) in 10 guinea pigs. Four guinea pigs were served as a control group. The other 100 guinea pigs were subdivided into 5 groups (20 per group) and received a 10 µl intravitreal injection every 2 days for 4 times. Two groups were injected with 20 or 50 µg/ml Shh amino-terminal peptide (Shh-N) into the right eye and 0.1% bovine serum albumin into the other. FDM was induced in the right eyes of the three cyclopamine-treated groups and both eyes were injected with 50, 100, or 200 µg/ml cyclopamine. Retinoscopic refraction and eye dimensions were assessed on Day 14 of treatment. MMP-2 protein expression was determined in both scleras by western blotting. Both concentrations of Shh-N stimulated myopic development and axial growth as compared with control eyes. Myopia and axial elongation were significantly greater in the 50 µg/ml than in the 20 µg/ml Shh-N group (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0096952