In vitro synergistic antioxidant activity and identification of antioxidant components from Astragalus membranaceus and Paeonia lactiflora

Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membra...

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Bibliographic Details
Published in:PloS one 2014-05, Vol.9 (5), p.e96780
Main Authors: Xu, Xiaoyan, Li, Feng, Zhang, Xin, Li, Pengcheng, Zhang, Xing, Wu, Zhaoxi, Li, Dapeng
Format: Article
Language:English
Subjects:
Antioxidants
Antioxidants (Nutrients)
Apoptosis
Astragalus
Astragalus membranaceus - chemistry
Biological activity
Biology and Life Sciences
Biphenyl (Compound)
Biphenyl Compounds - chemistry
Cancer
Catalase
Catechin
Cell Line
Cell Survival - drug effects
Chromatography
Dietary supplements
Drug Synergism
Enzymes
Ferric ions
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - enzymology
Fibroblasts - metabolism
Flavonoids
Flavonoids - analysis
Food science
Fractionation
Free Radical Scavengers - chemistry
Free Radical Scavengers - pharmacology
Free radicals
Fruits
Glutathione
Glutathione peroxidase
Herbal medicine
Herbs
High performance liquid chromatography
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Liquid chromatography
Malondialdehyde - metabolism
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Oxidative Stress - drug effects
Paeonia - chemistry
Paeonia lactiflora
Peroxidase
Pharmacology
Phenol - analysis
Phenolic compounds
Phenols
Physical Sciences
Picrates - chemistry
Plant Extracts - chemistry
Plant Extracts - pharmacology
Quercetin
Recovery (Medical)
Rodents
Superoxide dismutase
Synergism
Synergistic effect
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Summary:Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membranaceus (AME) and Paeonia Lactiflora (PL), and identify the potential antioxidant components by 1,1-diphenyl-2-picrylhydrazine (DPPH) radical spiking test followed by a high performance liquid chromatography separation combined with diode array detection and tandem mass spectrometry analysis (DPPH-HPLC-DAD-MS/MS). Eight AME-PL combined extracts (E1-E8) were prepared based on bioactivity-guided fractionation. Among them, E1 exhibited the strongest synergistic effect in scavenging DPPH radicals and reducing ferric ions (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0096780