Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves

Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervent...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-05, Vol.9 (5), p.e98215-e98215
Main Authors: Zhang, Kewei, Liu, Hanhan, Tao, Peilin, Chen, Huan
Format: Article
Language:English
Subjects:
Antioxidants
Arabidopsis
Arabidopsis thaliana
Biology and Life Sciences
Biosynthesis
Calvin cycle
Carbohydrate metabolism
Carbohydrates
Carbon dioxide
Carbon Dioxide - metabolism
Corn
Education
Electron transport
Energy metabolism
Engineering
Gene expression
Germplasm
Humidity
Hydrologic cycle
Laboratories
Leaves
Life sciences
Lipid composition
Lipid Metabolism
Lipids
Metabolic pathways
Metabolism
Oryza
Oxygen
Oxygen Consumption
Peroxides
Phosphates
Phosphorus
Phosphorus - deficiency
Photorespiration
Photosynthesis
Physiological aspects
Physiology
Plant biochemistry
Plant growth
Plant Leaves - metabolism
Polyamines
Potassium phosphate
Potassium phosphates
Protein biosynthesis
Protein synthesis
Protein turnover
Proteins
Proteomics - methods
Reactive oxygen species
Reactive Oxygen Species - metabolism
Starch
Starch - biosynthesis
Starvation
Stress
Stress (Psychology)
Stress, Physiological
Stresses
Studies
Sucrose
Transcription factors
Transduction
Virulence
Zea mays - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93
cites cdi_FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93
container_end_page e98215
container_issue 5
container_start_page e98215
container_title PloS one
container_volume 9
creator Zhang, Kewei
Liu, Hanhan
Tao, Peilin
Chen, Huan
description Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervention group, -P) for 25 days. In total, 1,342 protein spots were detected on 2-DE maps and 15.43% had changed (P
doi_str_mv 10.1371/journal.pone.0098215
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1528074454</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A418127513</galeid><doaj_id>oai_doaj_org_article_3fb6fee2b3fa41999b7fd09e0f0c995d</doaj_id><sourcerecordid>A418127513</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93</originalsourceid><addsrcrecordid>eNqNk11r2zAUhs3YWLts_2BshsHYLpLpw7Klm0Ep-wgUCvu6FSfycaxgW6kkp-t-_ZQmLcnoxTCyxNHzvpKOdLLsJSUzyiv6YeVGP0A3W7sBZ4Qoyah4lJ1Sxdm0ZIQ_PhifZM9CWBEiuCzLp9kJK6SQnFSnWXvu-jV4iHaD-dq7iK63JofkfBMwbEMbW2M-4HVuh2CXbQxpEF3euet83bqQmh9DHqLHEPL0SxvaKu2Q92D_YN4hbDA8z5400AV8se8n2c_Pn36cf51eXH6Zn59dTE2pWJwKUXBmCgOKN3xhKtI0hEnDgUtaKYYgKVSMgICalqbECgUD4DWtpDByofgke73zXXcu6H2SgqaCSVIVRbKfZPMdUTtY6bW3Pfgb7cDq24DzSw0-WtOh5s2ibBDZgjdQUKXUompqopA0xCgl6uT1cb_auOixNjhED92R6fHMYFu9dBtdEM54IZLBu72Bd1cjhqh7Gwx2HQzoxtt9K8krInlC3_yDPny6PbWEdAA7NC6ta7am-qygkrJK0K3X7AEqfTWm608vqrEpfiR4fyRITMTfcQljCHr-_dv_s5e_jtm3B2yL0MU2uG6MNr2iY7DYgca7EDw290mmRG8L4i4belsQel8QSfbq8ILuRXcVwP8Ch_MH2w</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1528074454</pqid></control><display><type>article</type><title>Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Zhang, Kewei ; Liu, Hanhan ; Tao, Peilin ; Chen, Huan</creator><contributor>Beemster, Gerrit TS</contributor><creatorcontrib>Zhang, Kewei ; Liu, Hanhan ; Tao, Peilin ; Chen, Huan ; Beemster, Gerrit TS</creatorcontrib><description>Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervention group, -P) for 25 days. In total, 1,342 protein spots were detected on 2-DE maps and 15.43% had changed (P&lt;0.05; ≥1.5-fold) significantly in quantity between the +P and -P groups. These proteins are involved in several major metabolic pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, secondary metabolism, signal transduction, protein synthesis, cell rescue and cell defense and virulence. The results showed that the reduction in photosynthesis under low phosphorus treatment was due to the down-regulation of the proteins involved in CO2 enrichment, the Calvin cycle and the electron transport system. Electron transport and photosynthesis restrictions resulted in a large accumulation of peroxides. Maize has developed many different reactive oxygen species (ROS) scavenging mechanisms to cope with low phosphorus stress, including up-regulating its antioxidant content and antioxidase activity. After being subjected to phosphorus stress over a long period, maize may increase its internal phosphorus utilization efficiency by altering photorespiration, starch synthesis and lipid composition. These results provide important information about how maize responds to low phosphorus stress.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0098215</identifier><identifier>PMID: 24858307</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antioxidants ; Arabidopsis ; Arabidopsis thaliana ; Biology and Life Sciences ; Biosynthesis ; Calvin cycle ; Carbohydrate metabolism ; Carbohydrates ; Carbon dioxide ; Carbon Dioxide - metabolism ; Corn ; Education ; Electron transport ; Energy metabolism ; Engineering ; Gene expression ; Germplasm ; Humidity ; Hydrologic cycle ; Laboratories ; Leaves ; Life sciences ; Lipid composition ; Lipid Metabolism ; Lipids ; Metabolic pathways ; Metabolism ; Oryza ; Oxygen ; Oxygen Consumption ; Peroxides ; Phosphates ; Phosphorus ; Phosphorus - deficiency ; Photorespiration ; Photosynthesis ; Physiological aspects ; Physiology ; Plant biochemistry ; Plant growth ; Plant Leaves - metabolism ; Polyamines ; Potassium phosphate ; Potassium phosphates ; Protein biosynthesis ; Protein synthesis ; Protein turnover ; Proteins ; Proteomics - methods ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Starch ; Starch - biosynthesis ; Starvation ; Stress ; Stress (Psychology) ; Stress, Physiological ; Stresses ; Studies ; Sucrose ; Transcription factors ; Transduction ; Virulence ; Zea mays - metabolism</subject><ispartof>PloS one, 2014-05, Vol.9 (5), p.e98215-e98215</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 zhang et al 2014 zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93</citedby><cites>FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1528074454/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1528074454?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24858307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Beemster, Gerrit TS</contributor><creatorcontrib>Zhang, Kewei</creatorcontrib><creatorcontrib>Liu, Hanhan</creatorcontrib><creatorcontrib>Tao, Peilin</creatorcontrib><creatorcontrib>Chen, Huan</creatorcontrib><title>Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervention group, -P) for 25 days. In total, 1,342 protein spots were detected on 2-DE maps and 15.43% had changed (P&lt;0.05; ≥1.5-fold) significantly in quantity between the +P and -P groups. These proteins are involved in several major metabolic pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, secondary metabolism, signal transduction, protein synthesis, cell rescue and cell defense and virulence. The results showed that the reduction in photosynthesis under low phosphorus treatment was due to the down-regulation of the proteins involved in CO2 enrichment, the Calvin cycle and the electron transport system. Electron transport and photosynthesis restrictions resulted in a large accumulation of peroxides. Maize has developed many different reactive oxygen species (ROS) scavenging mechanisms to cope with low phosphorus stress, including up-regulating its antioxidant content and antioxidase activity. After being subjected to phosphorus stress over a long period, maize may increase its internal phosphorus utilization efficiency by altering photorespiration, starch synthesis and lipid composition. These results provide important information about how maize responds to low phosphorus stress.</description><subject>Antioxidants</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Calvin cycle</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Corn</subject><subject>Education</subject><subject>Electron transport</subject><subject>Energy metabolism</subject><subject>Engineering</subject><subject>Gene expression</subject><subject>Germplasm</subject><subject>Humidity</subject><subject>Hydrologic cycle</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Life sciences</subject><subject>Lipid composition</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Oryza</subject><subject>Oxygen</subject><subject>Oxygen Consumption</subject><subject>Peroxides</subject><subject>Phosphates</subject><subject>Phosphorus</subject><subject>Phosphorus - deficiency</subject><subject>Photorespiration</subject><subject>Photosynthesis</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Plant biochemistry</subject><subject>Plant growth</subject><subject>Plant Leaves - metabolism</subject><subject>Polyamines</subject><subject>Potassium phosphate</subject><subject>Potassium phosphates</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Protein turnover</subject><subject>Proteins</subject><subject>Proteomics - methods</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Starch</subject><subject>Starch - biosynthesis</subject><subject>Starvation</subject><subject>Stress</subject><subject>Stress (Psychology)</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Studies</subject><subject>Sucrose</subject><subject>Transcription factors</subject><subject>Transduction</subject><subject>Virulence</subject><subject>Zea mays - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11r2zAUhs3YWLts_2BshsHYLpLpw7Klm0Ep-wgUCvu6FSfycaxgW6kkp-t-_ZQmLcnoxTCyxNHzvpKOdLLsJSUzyiv6YeVGP0A3W7sBZ4Qoyah4lJ1Sxdm0ZIQ_PhifZM9CWBEiuCzLp9kJK6SQnFSnWXvu-jV4iHaD-dq7iK63JofkfBMwbEMbW2M-4HVuh2CXbQxpEF3euet83bqQmh9DHqLHEPL0SxvaKu2Q92D_YN4hbDA8z5400AV8se8n2c_Pn36cf51eXH6Zn59dTE2pWJwKUXBmCgOKN3xhKtI0hEnDgUtaKYYgKVSMgICalqbECgUD4DWtpDByofgke73zXXcu6H2SgqaCSVIVRbKfZPMdUTtY6bW3Pfgb7cDq24DzSw0-WtOh5s2ibBDZgjdQUKXUompqopA0xCgl6uT1cb_auOixNjhED92R6fHMYFu9dBtdEM54IZLBu72Bd1cjhqh7Gwx2HQzoxtt9K8krInlC3_yDPny6PbWEdAA7NC6ta7am-qygkrJK0K3X7AEqfTWm608vqrEpfiR4fyRITMTfcQljCHr-_dv_s5e_jtm3B2yL0MU2uG6MNr2iY7DYgca7EDw290mmRG8L4i4belsQel8QSfbq8ILuRXcVwP8Ch_MH2w</recordid><startdate>20140523</startdate><enddate>20140523</enddate><creator>Zhang, Kewei</creator><creator>Liu, Hanhan</creator><creator>Tao, Peilin</creator><creator>Chen, Huan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140523</creationdate><title>Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves</title><author>Zhang, Kewei ; Liu, Hanhan ; Tao, Peilin ; Chen, Huan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antioxidants</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Calvin cycle</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Carbon dioxide</topic><topic>Carbon Dioxide - metabolism</topic><topic>Corn</topic><topic>Education</topic><topic>Electron transport</topic><topic>Energy metabolism</topic><topic>Engineering</topic><topic>Gene expression</topic><topic>Germplasm</topic><topic>Humidity</topic><topic>Hydrologic cycle</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Life sciences</topic><topic>Lipid composition</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Oryza</topic><topic>Oxygen</topic><topic>Oxygen Consumption</topic><topic>Peroxides</topic><topic>Phosphates</topic><topic>Phosphorus</topic><topic>Phosphorus - deficiency</topic><topic>Photorespiration</topic><topic>Photosynthesis</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Plant biochemistry</topic><topic>Plant growth</topic><topic>Plant Leaves - metabolism</topic><topic>Polyamines</topic><topic>Potassium phosphate</topic><topic>Potassium phosphates</topic><topic>Protein biosynthesis</topic><topic>Protein synthesis</topic><topic>Protein turnover</topic><topic>Proteins</topic><topic>Proteomics - methods</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Starch</topic><topic>Starch - biosynthesis</topic><topic>Starvation</topic><topic>Stress</topic><topic>Stress (Psychology)</topic><topic>Stress, Physiological</topic><topic>Stresses</topic><topic>Studies</topic><topic>Sucrose</topic><topic>Transcription factors</topic><topic>Transduction</topic><topic>Virulence</topic><topic>Zea mays - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Kewei</creatorcontrib><creatorcontrib>Liu, Hanhan</creatorcontrib><creatorcontrib>Tao, Peilin</creatorcontrib><creatorcontrib>Chen, Huan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale in Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Kewei</au><au>Liu, Hanhan</au><au>Tao, Peilin</au><au>Chen, Huan</au><au>Beemster, Gerrit TS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-05-23</date><risdate>2014</risdate><volume>9</volume><issue>5</issue><spage>e98215</spage><epage>e98215</epage><pages>e98215-e98215</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Phosphorus deficiency limits plant growth and development. To better understand the mechanisms behind how maize responds to phosphate stress, we compared the proteome analysis results of two groups of maize leaves that were treated separately with 1,000 µM (control, +P) and 5 µM of KH2PO4 (intervention group, -P) for 25 days. In total, 1,342 protein spots were detected on 2-DE maps and 15.43% had changed (P&lt;0.05; ≥1.5-fold) significantly in quantity between the +P and -P groups. These proteins are involved in several major metabolic pathways, including photosynthesis, carbohydrate metabolism, energy metabolism, secondary metabolism, signal transduction, protein synthesis, cell rescue and cell defense and virulence. The results showed that the reduction in photosynthesis under low phosphorus treatment was due to the down-regulation of the proteins involved in CO2 enrichment, the Calvin cycle and the electron transport system. Electron transport and photosynthesis restrictions resulted in a large accumulation of peroxides. Maize has developed many different reactive oxygen species (ROS) scavenging mechanisms to cope with low phosphorus stress, including up-regulating its antioxidant content and antioxidase activity. After being subjected to phosphorus stress over a long period, maize may increase its internal phosphorus utilization efficiency by altering photorespiration, starch synthesis and lipid composition. These results provide important information about how maize responds to low phosphorus stress.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24858307</pmid><doi>10.1371/journal.pone.0098215</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2014-05, Vol.9 (5), p.e98215-e98215
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1528074454
source Publicly Available Content Database; PubMed Central
subjects Antioxidants
Arabidopsis
Arabidopsis thaliana
Biology and Life Sciences
Biosynthesis
Calvin cycle
Carbohydrate metabolism
Carbohydrates
Carbon dioxide
Carbon Dioxide - metabolism
Corn
Education
Electron transport
Energy metabolism
Engineering
Gene expression
Germplasm
Humidity
Hydrologic cycle
Laboratories
Leaves
Life sciences
Lipid composition
Lipid Metabolism
Lipids
Metabolic pathways
Metabolism
Oryza
Oxygen
Oxygen Consumption
Peroxides
Phosphates
Phosphorus
Phosphorus - deficiency
Photorespiration
Photosynthesis
Physiological aspects
Physiology
Plant biochemistry
Plant growth
Plant Leaves - metabolism
Polyamines
Potassium phosphate
Potassium phosphates
Protein biosynthesis
Protein synthesis
Protein turnover
Proteins
Proteomics - methods
Reactive oxygen species
Reactive Oxygen Species - metabolism
Starch
Starch - biosynthesis
Starvation
Stress
Stress (Psychology)
Stress, Physiological
Stresses
Studies
Sucrose
Transcription factors
Transduction
Virulence
Zea mays - metabolism
title Comparative proteomic analyses provide new insights into low phosphorus stress responses in maize leaves
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T13%3A12%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20proteomic%20analyses%20provide%20new%20insights%20into%20low%20phosphorus%20stress%20responses%20in%20maize%20leaves&rft.jtitle=PloS%20one&rft.au=Zhang,%20Kewei&rft.date=2014-05-23&rft.volume=9&rft.issue=5&rft.spage=e98215&rft.epage=e98215&rft.pages=e98215-e98215&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0098215&rft_dat=%3Cgale_plos_%3EA418127513%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-55432c4ca93f3bc70ff028c3a381792ea81a720a5ad16c6e7e52aa3d1785c8b93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1528074454&rft_id=info:pmid/24858307&rft_galeid=A418127513&rfr_iscdi=true