Milk oligosaccharides over time of lactation from different dog breeds

The partnership of humans and dogs goes back to over 10'000 years, yet relatively little is known about a dog's first extra-uterine nutrition particularly when it comes to milk oligosaccharides. We set out to identify and quantify milk oligosaccharides over the course of lactation from dif...

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Published in:PloS one 2014-06, Vol.9 (6), p.e99824-e99824
Main Authors: Macias Rostami, Shirin, Bénet, Thierry, Spears, Julie, Reynolds, Arleigh, Satyaraj, Ebenezer, Sprenger, Norbert, Austin, Sean
Format: Article
Language:English
Subjects:
Acids
Animals
Biology and Life Sciences
Breeding
Carbohydrates
Chromatography
Chromatography, High Pressure Liquid
Dogs
Female
Fluorescence
Genomes
Hexose
Hexoses
Lactation
Lactation - metabolism
Lactose
Lactose - analysis
Lactose - metabolism
Limit of Detection
Mass spectrometry
Mass spectroscopy
Microbiota
Milk
Milk - chemistry
Milk - metabolism
Monosaccharides
Nutrition
Nutritional requirements
Oligosaccharides
Oligosaccharides - analysis
Oligosaccharides - metabolism
Physical Sciences
Prebiotics
Research and Analysis Methods
Species Specificity
Time Factors
Uterus
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cited_by cdi_FETCH-LOGICAL-c762t-5c6b47a8c7d6d7f144b991437d41a73c7f9bdfe102bfbe5783bd9a50aff11b203
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creator Macias Rostami, Shirin
Bénet, Thierry
Spears, Julie
Reynolds, Arleigh
Satyaraj, Ebenezer
Sprenger, Norbert
Austin, Sean
description The partnership of humans and dogs goes back to over 10'000 years, yet relatively little is known about a dog's first extra-uterine nutrition particularly when it comes to milk oligosaccharides. We set out to identify and quantify milk oligosaccharides over the course of lactation from different dog breeds (Labrador retriever, Schnauzer and 3 Alaskan husky crossbreeds). To this end, 2 different chromatographic methods with fluorescence and mass spectrometry detection were developed and one was validated for quantification. Besides lactose and lactose-sulphate, we identified 2 different trisaccharides composed of 3 hexose units, 3'sialyllactose (3'SL), 6'sialyllactose (6'SL), 2'fucosyllactose (2'FL), and a tetrasaccharide composed of 2 hexoses, an N-acetylhexosamine and a deoxyhexose. 3'SL was present at the highest levels in milk of all dog breeds starting at around 7.5 g/L and dropping to about 1.5 g/L in the first 10 days of lactation. 6'SL was about 10 times less abundant and 2'FL and the tetrasaccharide had rather varying levels in the milk of the different breeds with the tetrasaccharide only detectable in the Alaskan husky crossbreeds. The longitudinal and quantitative data of milk oligosaccharides from different dog breeds are an important basis to further our understanding on their specific biological roles and also on the specific nutritional requirements of lactating puppies.
doi_str_mv 10.1371/journal.pone.0099824
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subjects Acids
Animals
Biology and Life Sciences
Breeding
Carbohydrates
Chromatography
Chromatography, High Pressure Liquid
Dogs
Female
Fluorescence
Genomes
Hexose
Hexoses
Lactation
Lactation - metabolism
Lactose
Lactose - analysis
Lactose - metabolism
Limit of Detection
Mass spectrometry
Mass spectroscopy
Microbiota
Milk
Milk - chemistry
Milk - metabolism
Monosaccharides
Nutrition
Nutritional requirements
Oligosaccharides
Oligosaccharides - analysis
Oligosaccharides - metabolism
Physical Sciences
Prebiotics
Research and Analysis Methods
Species Specificity
Time Factors
Uterus
title Milk oligosaccharides over time of lactation from different dog breeds
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