Pathogenicity of Shigella in chickens

Shigellosis in chickens was first reported in 2004. This study aimed to determine the pathogenicity of Shigella in chickens and the possibility of cross-infection between humans and chickens. The pathogenicity of Shigella in chickens was examined via infection of three-day-old SPF chickens with Shig...

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Bibliographic Details
Published in:PloS one 2014-06, Vol.9 (6), p.e100264-e100264
Main Authors: Shi, Run, Yang, Xia, Chen, Lu, Chang, Hong-tao, Liu, Hong-ying, Zhao, Jun, Wang, Xin-wei, Wang, Chuan-qing
Format: Article
Language:English
Subjects:
Ability tests
Adaptation
Animal sciences
Animals
Bacteria
Biology and Life Sciences
Chickens
Chickens - microbiology
Crop
Cross-infection
Electron microscopy
Epithelial cells
HeLa Cells
Humans
Immunohistochemistry
Infections
Injection
Intestinal Mucosa - microbiology
Intestine
Invasiveness
Laboratory animals
Medicine and Health Sciences
Membranes
Mucosa
Pathogenicity
Pathogens
Poultry
Public health
Shigella
Shigella - isolation & purification
Shigella - pathogenicity
Shigellosis
Specific Pathogen-Free Organisms
Transmission electron microscopy
Veterinary medicine
Virulence
Zoology
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Summary:Shigellosis in chickens was first reported in 2004. This study aimed to determine the pathogenicity of Shigella in chickens and the possibility of cross-infection between humans and chickens. The pathogenicity of Shigella in chickens was examined via infection of three-day-old SPF chickens with Shigella strain ZD02 isolated from a human patient. The virulence and invasiveness were examined by infection of the chicken intestines and primary chicken intestinal epithelial cells. The results showed Shigella can cause death via intraperitoneal injection in SPF chickens, but only induce depression via crop injection. Immunohistochemistry and transmission electron microscopy revealed the Shigella can invade the intestinal epithelia. Immunohistochemistry of the primary chicken intestinal epithelial cells infected with Shigella showed the bacteria were internalized into the epithelial cells. Electron microscopy also confirmed that Shigella invaded primary chicken intestinal epithelia and was encapsulated by phagosome-like membranes. Our data demonstrate that Shigella can invade primary chicken intestinal epithelial cells in vitro and chicken intestinal mucosa in vivo, resulting in pathogenicity and even death. The findings suggest Shigella isolated from human or chicken share similar pathogenicity as well as the possibility of human-poultry cross-infection, which is of public health significance.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0100264