The complete nucleotide sequence of the mitochondrial genome of Bactrocera minax (Diptera: Tephritidae)

• Published in: PloS one 2014-06, Vol.9 (6), p.e100558
• Main Authors: Zhang, Bin, Nardi, Francesco, Hull-Sanders, Helen, Wan, Xuanwu, Liu, Yinghong
• Format: Article
• Language: English
• Subjects: Amino acid sequence; Amino acids; Analysis; Animals; Bactrocera; Bactrocera carambolae; Bactrocera cucurbitae; Bactrocera dorsalis; Bactrocera minax; Bactrocera oleae; Bactrocera papayae; Bactrocera philippinensis; Bactrocera tryoni; Base Sequence; Biology; Biology and Life Sciences; Citrus fruits; Conservation; Conserved sequence; Correlation; Correlation analysis; Cytochrome; Deoxyribonucleic acid; Diptera; DNA; DNA, Intergenic - genetics; Evolution; Fruit flies (Tephritidae); Genes; Genome, Mitochondrial - genetics; Genomes; Genomics; Homology; Insect Proteins - genetics; Insects; Mitochondria; Mitochondrial DNA; Mutation; Nucleotide sequence; Nucleotides - genetics; Orthoptera; Phylogenetics; Phylogeography; Population genetics; RNA, Ribosomal - genetics; RNA, Transfer - genetics; Species; Studies; Substitutes; Tephritidae; Tephritidae - genetics; Tetradacus; Transfer RNA; Wildlife conservation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0100558

The complete 16,043 bp mitochondrial genome (mitogenome) of Bactrocera minax (Diptera: Tephritidae) has been sequenced. The genome encodes 37 genes usually found in insect mitogenomes. The mitogenome information for B. minax was compared to the homologous sequences of Bactrocera oleae, Bactrocera tryoni, Bactrocera philippinensis, Bactrocera carambolae, Bactrocera papayae, Bactrocera dorsalis, Bactrocera correcta, Bactrocera cucurbitae and Ceratitis capitata. The analysis indicated the structure and organization are typical of, and similar to, the nine closely related species mentioned above, although it contains the lowest genome-wide A+T content (67.3%). Four short intergenic spacers with a high degree of conservation among the nine tephritid species mentioned above and B. minax were observed, which also have clear counterparts in the control regions (CRs). Correlation analysis among these ten tephritid species revealed close positive correlation between the A+T content of zero-fold degenerate sites (P0FD), the ratio of nucleotide substitution frequency at P0FD sites to all degenerate sites (zero-fold degenerate sites, two-fold degenerate sites and four-fold degenerate sites) and amino acid sequence distance (ASD) were found. Further, significant positive correlation was observed between the A+T content of four-fold degenerate sites (P4FD) and the ratio of nucleotide substitution frequency at P4FD sites to all degenerate sites; however, we found significant negative correlation between ASD and the A+T content of P4FD, and the ratio of nucleotide substitution frequency at P4FD sites to all degenerate sites. A higher nucleotide substitution frequency at non-synonymous sites compared to synonymous sites was observed in nad4, the first time that has been observed in an insect mitogenome. A poly(T) stretch at the 5' end of the CR followed by a [TA(A)]n-like stretch was also found. In addition, a highly conserved G+A-rich sequence block was observed in front of the poly(T) stretch among the ten tephritid species and two tandem repeats were present in the CR.