Metallothionein 2 (SaMT2) from Sedum alfredii Hance confers increased Cd tolerance and accumulation in yeast and tobacco

Metallothioneins are cysteine-rich metal-binding proteins. In the present study, SaMT2, a type 2 metallothionein gene, was isolated from Cd/Zn co-hyperaccumulator Sedum alfredii Hance. SaMT2 encodes a putative peptide of 79 amino acid residues including two cysteine-rich domains. The transcript leve...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-07, Vol.9 (7), p.e102750
Main Authors: Zhang, Jie, Zhang, Min, Tian, Shengke, Lu, Lingli, Shohag, M J I, Yang, Xiaoe
Format: Article
Language:English
Subjects:
Accumulation
Amino acids
Antioxidants
Antioxidants - metabolism
Arabidopsis
Arabidopsis thaliana
Biology and Life Sciences
Cadmium
Cadmium - metabolism
Cajanus cajan
Chelation
Cloning
Cysteine
Cysteine - metabolism
E coli
Ecology and Environmental Sciences
Ecosystem biology
Ecosystems
Ectopic expression
Escherichia coli
Gene Expression Regulation, Plant - physiology
Genes
Genetic engineering
Genetically modified plants
Homeostasis
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Laboratories
Metallothionein
Metallothionein - metabolism
Metallothioneins
Metals
Molecular biology
Nicotiana - metabolism
Oryza
Physiology
Plant Roots - metabolism
Plant sciences
Plant Shoots - metabolism
Plants, Genetically Modified - metabolism
Plasmids
Protein binding
Proteins
Roots
Sedum - metabolism
Sedum alfredii
Shoots
Thlaspi caerulescens
Tobacco
Transcription
Transgenic plants
Yeast
Yeasts - metabolism
Zinc
Zinc - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Metallothioneins are cysteine-rich metal-binding proteins. In the present study, SaMT2, a type 2 metallothionein gene, was isolated from Cd/Zn co-hyperaccumulator Sedum alfredii Hance. SaMT2 encodes a putative peptide of 79 amino acid residues including two cysteine-rich domains. The transcript level of SaMT2 was higher in shoots than in roots of S. alfredii, and was significantly induced by Cd and Zn treatments. Yeast expression assay showed SaMT2 significantly enhanced Cd tolerance and accumulation in yeast. Ectopic expression of SaMT2 in tobacco enhanced Cd and Zn tolerance and accumulation in both shoots and roots of the transgenic plants. The transgenic plants had higher antioxidant enzyme activities and accumulated less H2O2 than wild-type plants under Cd and Zn treatment. Thus, SaMT2 could significantly enhance Cd and Zn tolerance and accumulation in transgenic tobacco plants by chelating metals and improving antioxidant system.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0102750