PGE₂ promotes apoptosis induced by cytokine deprivation through EP3 receptor and induces Bim in mouse mast cells

• Published in: PloS one 2014-07, Vol.9 (7), p.e102948-e102948
• Main Authors: Kovarova, Martina, Koller, Beverly H
• Format: Article
• Language: English
• Subjects: Activation; Animals; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Apoptosis Regulatory Proteins - metabolism; Bcl-2-Like Protein 11; BIM protein; Biology and Life Sciences; Calcium; Calcium (intracellular); Calcium - metabolism; Calcium-binding protein; Calmodulin; Caspase; Caspase-3; Cell death; Cell survival; Chromatin; Cytokines; Cytokines - metabolism; Deoxyribonucleic acid; Deprivation; Dinoprostone - pharmacology; DNA; Hypersensitivity; Inflammation; Kinases; Laboratory animals; Ligands; Low molecular weights; MAP kinase; Mast cells; Mast Cells - drug effects; Mast Cells - metabolism; Membrane Proteins - metabolism; Mice; Mice, Knockout; Mitochondria; Molecular weight; Mortality; Neutrophils; Phosphorylation; Prostaglandin E2; Prostaglandins; Proteins; Proto-Oncogene Proteins - metabolism; Receptors; Receptors, Prostaglandin E, EP3 Subtype - metabolism; Restoration; Rodents; Signal Transduction - drug effects; Signal Transduction - physiology; Tissues
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0102948

Increased mast cell numbers are observed at sites of allergic inflammation and restoration of normal mast cell numbers is critical to the resolution of these responses. Early studies showed that cytokines protect mast cells from apoptosis, suggesting a simple model in which diminished cytokine levels during resolution leads to cell death. The report that prostaglandins can contribute both to recruitment and to the resolution of inflammation together with the demonstration that mast cells express all four PGE2 receptors raises the question of whether a single PGE2 receptor mediates the ability of PGE2 to regulate mast cell survival and apoptosis. We report here that PGE2 through the EP3 receptor promotes cell death of mast cells initiated by cytokine withdrawal. Furthermore, the ability of PGE2 to limit reconstitution of tissues with cultured mast cells is lost in cell lacking the EP3 receptor. Apoptosis is accompanied by higher dissipation of mitochondrial potential (ΔΨm), increased caspase-3 activation, chromatin condensation, and low molecular weight DNA cleavage. PGE2 augmented cell death is dependent on an increase in intracellular calcium release, calmodulin dependent kinase II and MAPK activation. Synergy between the EP3 pathway and the intrinsic mitochondrial apoptotic pathway results in increased Bim expression and higher sensitivity of mast cells to cytokine deprivation. This supports a model in which PGE2 can contribute to the resolution of inflammation in part by augmenting the removal of inflammatory cells in this case, mast cells.