Effects of nitrogen and phosphorus fertilization on soil carbon fractions in alpine meadows on the Qinghai-Tibetan Plateau

• Published in: PloS one 2014-07, Vol.9 (7), p.e103266-e103266
• Main Authors: Li, Jin Hua, Yang, Yu Jie, Li, Bo Wen, Li, Wen Jin, Wang, Gang, Knops, Johannes M H
• Format: Article
• Language: English
• Subjects: Alpine environments; Biology and Life Sciences; Biomass; Carbon - chemistry; Carbon sequestration; Comparative analysis; Ecology and Environmental Sciences; Ecosystem; Ecosystems; Enzymes; Evaluation; Fertilization; Fertilizers; Functional groups; Grasses; Grassland; Grasslands; Kobresia; Laboratories; Legumes; Life sciences; Meadows; Microbial activity; Microorganisms; Mineralization; Nitrogen; Nitrogen - chemistry; Organic soils; Phosphorus; Phosphorus - chemistry; Plant biomass; Plants; Respiratory quotient; Sea level; Soil - chemistry; Soil carbon; Soil Microbiology; Soil testing; Soils; Substrates; Taiga & tundra; Tibet
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0103266

In grassland ecosystems, N and P fertilization often increase plant productivity, but there is no concensus if fertilization affects soil C fractions. We tested effects of N, P and N+P fertilization at 5, 10, 15 g m-2 yr-1 (N5, N10, N15, P5, P10, P15, N5P5, N10P10, and N15P15) compared to unfertilized control on soil C, soil microbial biomass and functional diversity at the 0-20 cm and 20-40 cm depth in an alpine meadow after 5 years of continuous fertilization. Fertilization increased total aboveground biomass of community and grass but decreased legume and forb biomass compared to no fertilization. All fertilization treatments decreased the C:N ratios of legumes and roots compared to control, however fertilization at rates of 5 and 15 g m-2 yr-1 decreased the C:N ratios of the grasses. Compared to the control, soil microbial biomass C increased in N5, N10, P5, and P10 in 0-20 cm, and increased in N10 and P5 while decreased in other treatments in 20-40 cm. Most of the fertilization treatments decreased the respiratory quotient (qCO2) in 0-20 cm but increased qCO2 in 20-40 cm. Fertilization increased soil microbial functional diversity (except N15) but decreased cumulative C mineralization (except in N15 in 0-20 cm and N5 in 20-40 cm). Soil organic C (SOC) decreased in P5 and P15 in 0-20 cm and for most of the fertilization treatments (except N15P15) in 20-40 cm. Overall, these results suggested that soils will not be a C sink (except N15P15). Nitrogen and phosphorus fertilization may lower the SOC pool by altering the plant biomass composition, especially the C:N ratios of different plant functional groups, and modifying C substrate utilization patterns of soil microbial communities. The N+P fertilization at 15 g m-2 yr-1 may be used in increasing plant aboveground biomass and soil C accumulation under these meadows.