An arched micro-injector (ARCMI) for innocuous subretinal injection

Several critical ocular diseases that can lead to blindness are due to retinal disorders. Subretinal drug delivery has been developed recently for the treatment of retinal disorders such as hemorrhage because of the specific ocular structure, namely, the blood retinal barrier (BRB). In the present s...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e104145
Main Authors: You, Yong Sung, Lee, Chang Yeol, Li, Chengguo, Lee, Sung Ho, Kim, Kibom, Jung, Hyungil
Format: Article
Language:English
Subjects:
Air flow
Airflow
Animals
Biology and Life Sciences
Biomimetics
Biotechnology
Blindness
Choroid - cytology
Curvature
Damage
Disorders
Drug delivery
Drug delivery systems
Electroplating
Equipment Design
Hemorrhage
Humans
Injection
Injectors
Macular degeneration
Mechanical Phenomena
Medicine and Health Sciences
Microinjections - adverse effects
Microinjections - instrumentation
Retina
Retina - cytology
Swine
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Summary:Several critical ocular diseases that can lead to blindness are due to retinal disorders. Subretinal drug delivery has been developed recently for the treatment of retinal disorders such as hemorrhage because of the specific ocular structure, namely, the blood retinal barrier (BRB). In the present study, we developed an Arched Micro-injector (ARCMI) for subretinal drug delivery with minimal retinal tissue damage. ARCMIs were fabricated using three major techniques: reverse drawing lithography, controlled air flow, and electroplating. In order to achieve minimal retinal tissue damage, ARCMIs were fabricated with specific features such as a 0.15 mm(-1) curvature, 45° tip bevel, 5 mm length, inner diameter of 40 µm, and an outer diameter of 100 µm. These specific features were optimized via in-vitro experiments in artificial ocular hemispherical structures and subretinal injection of indocyanine green in porcine eye ex-vivo. We confirmed that the ARCMI was capable of delivering ocular drugs by subretinal injection without unusual subretinal tissue damage, including hemorrhage.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0104145