Successful non-surgical deep uterine transfer of porcine morulae after 24 hour culture in a chemically defined medium

Excellent fertility and prolificacy have been reported after non-surgical deep uterine transfers of fresh in vivo-derived porcine embryos. Unfortunately, when this technology is used with vitrified embryos, the reproductive performance of recipients is low. For this reason and because the embryos mu...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e104696-e104696
Main Authors: Martinez, Emilio A, Angel, Miguel Angel, Cuello, Cristina, Sanchez-Osorio, Jonatan, Gomis, Jesus, Parrilla, Inmaculada, Vila, Jordi, Colina, Ignaci, Diaz, Marta, Reixach, Josep, Vazquez, Jose Luis, Vazquez, Juan Maria, Roca, Jordi, Gil, Maria Antonia
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biology and Life Sciences
Blastocysts
Bovine serum albumin
Breeding - methods
Carbon dioxide
Cell cycle
Culture
Culture media
Culture Media - chemistry
Disease transmission
Embryo Culture Techniques - methods
Embryo Culture Techniques - veterinary
Embryo transfer
Embryo Transfer - methods
Embryo Transfer - veterinary
Embryonic development
Embryos
Farms
Female
Fertility
Fetal calf serum
Hogs
International trade
Media (culture)
Medicine
Medicine and Health Sciences
Morula - physiology
Pigs
Pregnancy
R&D
Reproduction
Research & development
Serum albumin
Short term
Surgery
Swine
Swine - physiology
Temperature
Uterus
Viability
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Summary:Excellent fertility and prolificacy have been reported after non-surgical deep uterine transfers of fresh in vivo-derived porcine embryos. Unfortunately, when this technology is used with vitrified embryos, the reproductive performance of recipients is low. For this reason and because the embryos must be stored until they are transferred to the recipient farms, we evaluated the potential application of non-surgical deep uterine transfers with in vivo-derived morulae cultured for 24 h in liquid stage. In Experiment 1, two temperatures (25 °C and 37 °C) and two media (one fully defined and one semi-defined) were assessed. Morulae cultured in culture medium supplemented with bovine serum albumin and fetal calf serum at 38.5 °C in 5% CO2 in air were used as controls. Irrespective of medium, the embryo viability after 24 h of culture was negatively affected (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0104696