A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application

A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachmen...

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Bibliographic Details
Published in:PloS one 2014-08, Vol.9 (8), p.e104389-e104389
Main Authors: Balaji Raghavendran, Hanumantha Rao, Puvaneswary, Subramaniam, Talebian, Sepehr, Murali, Malliga R, Raman Murali, Malliga, Naveen, Sangeetha V, Vasudevaraj Naveen, Sangeetha, Krishnamurithy, G, McKean, Robert, Kamarul, Tunku
Format: Article
Language:English
Subjects:
Apoptosis
Biochemistry
Biocompatibility
Biology and Life Sciences
Biomedical materials
Bone healing
Bone marrow
Calcium
Cell adhesion
Cell adhesion & migration
Cell growth
Cells, Cultured
Chemical composition
Collagen
Comparative studies
Cytology
Data processing
Differentiation (biology)
Durapatite
Electrons
Electrospinning
Extracellular matrix
Fibronectin
Fibrous structure
Gene expression
Growth factors
Humans
Hydroxyapatite
Kinases
Localization
Matrix protein
Medicine
Mesenchymal Stromal Cells - cytology
Mesenchyme
Mineralization
Osteocalcin
Osteogenesis - physiology
Physical Sciences
Physiology
Polyesters
Polylactic acid
Priming
Proteins
Scaffolds
Stem cells
Stromal cells
Surface roughness
Surgery
Surgical implants
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds
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Summary:A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs) differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200-950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0104389