Anti-obesity effects of granulocyte-colony stimulating factor in Otsuka-Long-Evans-Tokushima fatty rats

• Published in: PloS one 2014-08, Vol.9 (8), p.e105603
• Main Authors: Lee, Yonggu, Song, Yi-Sun, Fang, Cheng-Hu, So, Byung-Im, Park, Jun-Young, Joo, Hyun-Woo, Park, In-Hwa, Shen, Guang-Yin, Shin, Jeong-Hun, Kim, Hyuck, Ahn, You-Heon, Kim, Kyung-Soo
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipocytes, Brown - metabolism; Adipose tissue; Adipose tissue (brown); Adipose Tissue - metabolism; Adipose Tissue - physiopathology; Animals; Apoptosis; Appetite; Biology and Life Sciences; Body fat; Body weight; Cardiology; Cells, Cultured; Cerebrospinal fluid; Cholesterol; Cholesterol - blood; Ciliary neurotrophic factor; Colonies; Colony-stimulating factor; Cytokines; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - physiopathology; Diabetic neuropathy; Energy; Energy balance; Energy expenditure; Engineering; Food; Food intake; Gene Expression Regulation - drug effects; Granulocyte colony-stimulating factor; Granulocyte Colony-Stimulating Factor - pharmacology; Homeostasis; Immunofluorescence; Immunomodulation; Inflammation; Insulin resistance; Interleukin 6; Interleukin-6 - blood; Internal medicine; Intracellular signalling; Ion Channels - biosynthesis; Kinases; Leptin; Leukocytes (granulocytic); Male; MAP kinase; Medical research; Medicine; Medicine and Health Sciences; Metabolism; Mitochondrial Proteins - biosynthesis; Obesity; Obesity - blood; Obesity - drug therapy; Obesity - physiopathology; Protein kinase; Proteins; Rats; Rats, Inbred OLETF; Receptors; Research and Analysis Methods; Rodents; Science; Thoracic surgery; Time Factors; Triglycerides; Tumor Necrosis Factor-alpha - blood; Uncoupling Protein 1; Weight control; Weight reduction
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0105603

Granulocyte-colony stimulating factor (G-CSF) has molecular structures and intracellular signaling pathways that are similar to those of leptin and ciliary neurotropic factor (CNTF). It also has immune-modulatory properties. Given that leptin and CNTF play important roles in energy homeostasis and that obesity is an inflammatory condition in adipose tissue, we hypothesized that G-CSF could also play a role in energy homeostasis. We treated 12 38-week-old male Otsuka-Long-Evans-Tokushima fatty rats (OLETF, diabetic) and 12 age-matched male Long-Evans-Tokushima rats (LETO, healthy) with 200 µg/day G-CSF or saline for 5 consecutive days. Body weight reduction was greater in G-CSF-treated OLETF (G-CSF/OLETF) than saline-treated OLETF (saline/OLETF) following 8 weeks of treatment (-6.9±1.6% vs. -3.1±2.2%, p