TLR ligands induce antiviral responses in chicken macrophages

Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immuni...

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Published in:PloS one 2014-08, Vol.9 (8), p.e105713-e105713
Main Authors: Barjesteh, Neda, Behboudi, Shahriar, Brisbin, Jennifer T, Villanueva, Alexander Ian, Nagy, Eva, Sharif, Shayan
Format: Article
Language:English
Subjects:
Animals
Avian flu
Avian influenza
Avian influenza viruses
Biological response modifiers
Biology and Life Sciences
Cell activation
Cell Line
Chickens
CpG islands
Gene expression
Genes
Health aspects
IL-1β
Imidazoles - pharmacology
Immune system
Immunity
Inflammation
Influenza
Influenza A virus
Influenza in Birds - virology
Interferon
Interferon regulatory factor
Interleukins
Kinases
Ligands
Lipopeptides - pharmacology
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Macrophages
Macrophages - drug effects
Macrophages - immunology
Oligodeoxyribonucleotides - pharmacology
Oligonucleotides
Orthomyxoviridae
Pandemics
Pathogens
Poly I-C - pharmacology
Polyinosinic:polycytidylic acid
Poultry
Proteins
Receptors
Replication
T cell receptors
TLR2 protein
Toll-like receptors
Toll-Like Receptors - agonists
Virus replication
Virus Replication - drug effects
Virus Replication - immunology
Viruses
β-Interferon
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cited_by cdi_FETCH-LOGICAL-c692t-1930c09c1bc731a2b2b4388650fea73c68b883800583ab9493bc15ad8af27c433
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container_issue 8
container_start_page e105713
container_title PloS one
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creator Barjesteh, Neda
Behboudi, Shahriar
Brisbin, Jennifer T
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Nagy, Eva
Sharif, Shayan
description Chicken macrophages express several receptors for recognition of pathogens, including Toll-like receptors (TLRs). TLRs bind to pathogen-associated molecular patterns (PAMPs) derived from bacterial or viral pathogens leading to the activation of macrophages. Macrophages play a critical role in immunity against viruses, including influenza viruses. The present study was designed to test the hypothesis that treatment of chicken macrophages with TLR ligands reduces avian influenza replication. Furthermore, we sought to study the expression of some of the key mediators involved in the TLR-mediated antiviral responses of macrophages. Chicken macrophages were treated with the TLR2, 3, 4, 7 and 21 ligands, Pam3CSK4, poly(I:C), LPS, R848 and CpG ODN, respectively, at different doses and time points pre- and post-H4N6 avian influenza virus (AIV) infection. The results revealed that pre-treatment of macrophages with Pam3CSK4, LPS and CpG ODN reduced the replication of AIV in chicken macrophages. In addition, the relative expression of genes involved in inflammatory and antiviral responses were quantified at 3, 8 and 18 hours post-treatment with the TLR2, 4 and 21 ligands. Pam3CSK4, LPS and CpG ODN increased the expression of interleukin (IL)-1β, interferon (IFN)-γ, IFN-β and interferon regulatory factor (IFR) 7. The expression of these genes correlated with the reduction of viral replication in macrophages. These results shed light on the process of immunity to AIV in chickens.
doi_str_mv 10.1371/journal.pone.0105713
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identifier ISSN: 1932-6203
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issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1558100117
source Publicly Available Content Database; PubMed Central
subjects Animals
Avian flu
Avian influenza
Avian influenza viruses
Biological response modifiers
Biology and Life Sciences
Cell activation
Cell Line
Chickens
CpG islands
Gene expression
Genes
Health aspects
IL-1β
Imidazoles - pharmacology
Immune system
Immunity
Inflammation
Influenza
Influenza A virus
Influenza in Birds - virology
Interferon
Interferon regulatory factor
Interleukins
Kinases
Ligands
Lipopeptides - pharmacology
Lipopolysaccharides
Lipopolysaccharides - pharmacology
Macrophages
Macrophages - drug effects
Macrophages - immunology
Oligodeoxyribonucleotides - pharmacology
Oligonucleotides
Orthomyxoviridae
Pandemics
Pathogens
Poly I-C - pharmacology
Polyinosinic:polycytidylic acid
Poultry
Proteins
Receptors
Replication
T cell receptors
TLR2 protein
Toll-like receptors
Toll-Like Receptors - agonists
Virus replication
Virus Replication - drug effects
Virus Replication - immunology
Viruses
β-Interferon
title TLR ligands induce antiviral responses in chicken macrophages
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