Biopsy of human morula-stage embryos: outcome of 215 IVF/ICSI cycles with PGS

Preimplantation genetic diagnosis (PGD) is commonly performed on biopsies from 6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, respectively. Day 4 human embryos at the morula stage were successfully biopsied. Biopsy was performed on 709 morulae from 215 ICSI cycles with pr...

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Bibliographic Details
Published in:PloS one 2014-09, Vol.9 (9), p.e106433-e106433
Main Authors: Zakharova, Elena E, Zaletova, Victoria V, Krivokharchenko, Alexander S
Format: Article
Language:English
Subjects:
Adult
Aneuploidy
Babies
Biology and Life Sciences
Biopsy
Blastocysts
Chromosomes
Diagnostic systems
Embryo Transfer
Embryos
Female
Fertilization in Vitro
Fluorescence
Fluorescence in situ hybridization
Gene expression
Genetic screening
Genomes
Humans
Hybridization
In Situ Hybridization, Fluorescence
In vitro fertilization
Laboratories
Medicine
Medicine and Health Sciences
Morula - cytology
Patients
Polymerase chain reaction
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Preimplantation Diagnosis
Reproductive health
Screening
Sperm Injections, Intracytoplasmic
Trophectoderm
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Summary:Preimplantation genetic diagnosis (PGD) is commonly performed on biopsies from 6-8-cell-stage embryos or blastocyst trophectoderm obtained on day 3 or 5, respectively. Day 4 human embryos at the morula stage were successfully biopsied. Biopsy was performed on 709 morulae from 215 ICSI cycles with preimplantation genetic screening (PGS), and 3-7 cells were obtained from each embryo. The most common vital aneuploidies (chromosomes X/Y, 21) were screened by fluorescence in situ hybridization (FISH). No aneuploidy was observed in 72.7% of embryos, 91% of those developed to blastocysts. Embryos were transferred on days 5-6. Clinical pregnancy was obtained in 32.8% of cases, and 60 babies were born. Patients who underwent ICSI/PGS treatment were compared with those who underwent standard ICSI treatment by examining the percentage of blastocysts, pregnancy rate, gestational length, birth height and weight. No significant differences in these parameters were observed between the groups. Day 4 biopsy procedure does not adversely affect embryo development in vitro or in vivo. The increased number of cells obtained by biopsy of morulae might facilitate diagnostic screening. There is enough time after biopsy to obtain PGD results for embryo transfer on day 5-6 in the current IVF cycle.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0106433