Etanercept attenuates myocardial ischemia/reperfusion injury by decreasing inflammation and oxidative stress

• Published in: PloS one 2014-09, Vol.9 (9), p.e108024
• Main Authors: Yang, Mei, Chen, Jianchang, Zhao, Jing, Meng, Mei
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Apoptosis; Apoptosis - drug effects; Arthritis; Bioengineering; Cardiomyocytes; Creatine Kinase, MB Form - blood; Creatine Kinase, MB Form - metabolism; Creatinine; Critical care; Cytokines; Digital imaging; Disease Models, Animal; Etanercept; Gene expression; Gene Expression Regulation - drug effects; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Heart; Immunoglobulin G - administration & dosage; Immunoglobulin G - pharmacology; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Injuries; Ischemia; L-Lactate dehydrogenase; Laboratory animals; Lactate dehydrogenase; Lactate Dehydrogenases - metabolism; Lactic acid; Male; Malondialdehyde; Malondialdehyde - metabolism; Medicine and Health Sciences; Myocardial infarction; Myocardial Infarction - drug therapy; Myocardial Infarction - metabolism; Myocardial Infarction - pathology; Myocardial ischemia; Myocardial Reperfusion Injury - drug therapy; Myocardial Reperfusion Injury - genetics; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocardial Reperfusion Injury - physiopathology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Neutrophil Infiltration - drug effects; Neutrophils; Oxidative stress; Oxidative Stress - drug effects; Peroxidase; Psoriasis; Rats; Receptors, Tumor Necrosis Factor - administration & dosage; Reperfusion; Rodents; Superoxide dismutase; Superoxide Dismutase - metabolism; TNF inhibitors; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Troponin I - blood; Troponin I - metabolism; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Ventricular Dysfunction - drug therapy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0108024

The protective role of etanercept in myocardial ischemia/reperfusion is not well understood. The aim of this study was to investigate whether etanercept modulates neutrophil accumulation, TNF-α induction and oxidative stress in an ischemia/reperfusion injured rat heart model. Rats were randomly exposed to sham operation, myocardial ischemia/reperfusion (MI/R) alone, MI/R+ etanercept. The results demonstrated that compared to MI/R, etanercept reduced myocardial infarction area, myocardial myeloperoxidase (MPO) levels, serum creatinine kinase (CK) and lactate dehydrogenase (LDH) levels, and both serum and myocardial TNF-α production. Etanercept also markedly enhanced the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and reduced the level of malondialdehyde (MDA) in MI/R rats. In summary, our data suggested that etanercept has protective effects against MI/R injury in rats, which may be attributed to attenuating inflammation and oxidative stress.