Foxp3 expression in macrophages associated with RENCA tumors in mice

The transcription factor Foxp3 represents the most specific functional marker of CD4+ regulatory T cells (TRegs). However, previous reports have described Foxp3 expression in other cell types including some subsets of macrophages, although there are conflicting reports and Foxp3 expression in cells...

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Bibliographic Details
Published in:PloS one 2014-09, Vol.9 (9), p.e108670-e108670
Main Authors: Devaud, Christel, Yong, Carmen S M, John, Liza B, Westwood, Jennifer A, Duong, Connie P M, House, Colin M, Denoyer, Delphine, Li, Jason, Darcy, Phillip K, Kershaw, Michael H
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibodies, Monoclonal - immunology
B cells
Bioinformatics
Biology and Life Sciences
Cancer
CD4 antigen
Cell Line, Tumor
Cloning
Cytometry
Diphtheria
Diphtheria toxin
Ethics
Flow cytometry
Forkhead Transcription Factors - biosynthesis
Forkhead Transcription Factors - genetics
Foxp3 protein
Gene sequencing
Immunoglobulins
Immunology
Immunoregulation
Kidney cancer
Kidney Neoplasms - immunology
Kidneys
Lymphocytes
Lymphocytes T
Macrophages
Macrophages - immunology
Mice
Mice, Inbred BALB C
Mice, SCID
Monoclonal antibodies
Oncology
Ribonucleic acid
RNA
RNA, Messenger - biosynthesis
T cells
T-Lymphocytes, Regulatory - immunology
Transcription factors
Tumors
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Summary:The transcription factor Foxp3 represents the most specific functional marker of CD4+ regulatory T cells (TRegs). However, previous reports have described Foxp3 expression in other cell types including some subsets of macrophages, although there are conflicting reports and Foxp3 expression in cells other than Treg is not well characterized. We performed detailed investigations into Foxp3 expression in macrophages in the normal tissue and tumor settings. We detected Foxp3 protein in macrophages infiltrating mouse renal cancer tumors injected subcutaneously or in the kidney. Expression was demonstrated using flow cytometry and Western blot with two individual monoclonal antibodies. Further analyses confirmed Foxp3 expression in macrophages by RT PCR, and studies using ribonucleic acid-sequencing (RNAseq) demonstrated a previously unknown Foxp3 messenger (m)RNA transcript in tumor-associated macrophages. In addition, depletion of Foxp3+ cells using diphtheria toxin in Foxp3DTR mice reduced the frequency of type-2 macrophages (M2) in kidney tumors. Collectively, these results indicate that tumor-associated macrophages could express Foxp3.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0108670