De novo transcriptome of the Hemimetabolous German cockroach (Blattella germanica)

The German cockroach, Blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. This insect has long served as a model system for studies of insect biology, physiology and ecology. However, the lack of genome or transcriptome...

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Published in:PloS one 2014-09, Vol.9 (9), p.e106932-e106932
Main Authors: Zhou, Xiaojie, Qian, Kun, Tong, Ying, Zhu, Junwei Jerry, Qiu, Xinghui, Zeng, Xiaopeng
Format: Article
Language:English
Subjects:
Adelphocoris lineolatus
Aedes aegypti
Allergic diseases
Amino Acid Sequence
Animal behavior
Animals
Biological activity
Biology and Life Sciences
Blattaria
Blattella germanica
Chemoreception
Cockroaches
Cockroaches - genetics
Detoxification
DNA, Complementary - genetics
Drosophila
Drosophila melanogaster
Ecological monitoring
Ecology
Expressed Sequence Tags
Females
Gene expression
Gene sequencing
Genes
Genomes
Genomics
Immunity
Immunity, Innate - genetics
Insecticides
Insects
Males
Manduca sexta
Medicine and Health Sciences
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
RNA, Messenger - genetics
RNA-mediated interference
Sequence Homology, Amino Acid
Simple sequence repeats
Solenopsis invicta
Transcriptome
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cited_by cdi_FETCH-LOGICAL-c592t-8e507bb35783dd2c27b680cecc114715ed1bd0c43f90273a4662f25f2941ab083
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container_issue 9
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creator Zhou, Xiaojie
Qian, Kun
Tong, Ying
Zhu, Junwei Jerry
Qiu, Xinghui
Zeng, Xiaopeng
description The German cockroach, Blattella germanica, is an important insect pest that transmits various pathogens mechanically and causes severe allergic diseases. This insect has long served as a model system for studies of insect biology, physiology and ecology. However, the lack of genome or transcriptome information heavily hinder our further understanding about the German cockroach in every aspect at a molecular level and on a genome-wide scale. To explore the transcriptome and identify unique sequences of interest, we subjected the B. germanica transcriptome to massively parallel pyrosequencing and generated the first reference transcriptome for B. germanica. A total of 1,365,609 raw reads with an average length of 529 bp were generated via pyrosequencing the mixed cDNA library from different life stages of German cockroach including maturing oothecae, nymphs, adult females and males. The raw reads were de novo assembled to 48,800 contigs and 3,961 singletons with high-quality unique sequences. These sequences were annotated and classified functionally in terms of BLAST, GO and KEGG, and the genes putatively coding detoxification enzyme systems, insecticide targets, key components in systematic RNA interference, immunity and chemoreception pathways were identified. A total of 3,601 SSRs (Simple Sequence Repeats) loci were also predicted. The whole transcriptome pyrosequencing data from this study provides a usable genetic resource for future identification of potential functional genes involved in various biological processes.
doi_str_mv 10.1371/journal.pone.0106932
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These sequences were annotated and classified functionally in terms of BLAST, GO and KEGG, and the genes putatively coding detoxification enzyme systems, insecticide targets, key components in systematic RNA interference, immunity and chemoreception pathways were identified. A total of 3,601 SSRs (Simple Sequence Repeats) loci were also predicted. 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subjects Adelphocoris lineolatus
Aedes aegypti
Allergic diseases
Amino Acid Sequence
Animal behavior
Animals
Biological activity
Biology and Life Sciences
Blattaria
Blattella germanica
Chemoreception
Cockroaches
Cockroaches - genetics
Detoxification
DNA, Complementary - genetics
Drosophila
Drosophila melanogaster
Ecological monitoring
Ecology
Expressed Sequence Tags
Females
Gene expression
Gene sequencing
Genes
Genomes
Genomics
Immunity
Immunity, Innate - genetics
Insecticides
Insects
Males
Manduca sexta
Medicine and Health Sciences
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
RNA, Messenger - genetics
RNA-mediated interference
Sequence Homology, Amino Acid
Simple sequence repeats
Solenopsis invicta
Transcriptome
title De novo transcriptome of the Hemimetabolous German cockroach (Blattella germanica)
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