Quantification of abdominal fat depots in rats and mice during obesity and weight loss interventions

Obesity is a leading healthcare issue contributing to metabolic diseases. There is a great interest in non-invasive approaches for quantitating abdominal fat in obese animals and humans. In this work, we propose an automated method to distinguish and quantify subcutaneous and visceral adipose tissue...

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Published in:PloS one 2014-10, Vol.9 (10), p.e108979-e108979
Main Authors: Kn, Bhanu Prakash, Gopalan, Venkatesh, Lee, Swee Shean, Velan, S Sendhil
Format: Article
Language:English
Subjects:
Abdomen
Abdominal Fat - pathology
Adipose tissue
Algorithms
Animal tissues
Animals
Automation
Biology and Life Sciences
Body Fat Distribution
Body weight loss
Consortia
Data processing
Diabetes
Diet, High-Fat
Engineering and Technology
Evolutionary algorithms
Fuzzy logic
Gene expression
Health care
High fat diet
Image processing
Image segmentation
Insulin resistance
Laboratories
Magnetic resonance
Magnetic Resonance Imaging
Medical imaging
Medicine and Health Sciences
Metabolic disorders
Mice
NMR
Nuclear magnetic resonance
Nutrient deficiency
Obesity
Obesity - pathology
Rats
Reproducibility of Results
Rodents
Science
Spine
Vertebrae
Weight control
Weight Loss
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Gopalan, Venkatesh
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description Obesity is a leading healthcare issue contributing to metabolic diseases. There is a great interest in non-invasive approaches for quantitating abdominal fat in obese animals and humans. In this work, we propose an automated method to distinguish and quantify subcutaneous and visceral adipose tissues (SAT and VAT) in rodents during obesity and weight loss interventions. We have also investigated the influence of different magnetic resonance sequences and sources of variability in quantification of fat depots. High-fat diet fed rodents were utilized for investigating the changes during obesity, exercise, and calorie restriction interventions (N = 7/cohort). Imaging was performed on a 7T Bruker ClinScan scanner using fast spin echo (FSE) and Dixon imaging methods to estimate the fat depots. Finally, we quantified the SAT and VAT volumes between the L1-L5 lumbar vertebrae using the proposed automatic hybrid geodesic region-based curve evolution algorithm. Significant changes in SAT and VAT volumes (p
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There is a great interest in non-invasive approaches for quantitating abdominal fat in obese animals and humans. In this work, we propose an automated method to distinguish and quantify subcutaneous and visceral adipose tissues (SAT and VAT) in rodents during obesity and weight loss interventions. We have also investigated the influence of different magnetic resonance sequences and sources of variability in quantification of fat depots. High-fat diet fed rodents were utilized for investigating the changes during obesity, exercise, and calorie restriction interventions (N = 7/cohort). Imaging was performed on a 7T Bruker ClinScan scanner using fast spin echo (FSE) and Dixon imaging methods to estimate the fat depots. Finally, we quantified the SAT and VAT volumes between the L1-L5 lumbar vertebrae using the proposed automatic hybrid geodesic region-based curve evolution algorithm. 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Significant changes in SAT and VAT volumes (p&lt;0.01) were observed between the pre- and post-intervention measurements. The SAT and VAT were 44.22±9%, 21.06±1.35% for control, -17.33±3.07%, -15.09±1.11% for exercise, and 18.56±2.05%, -3.9±0.96% for calorie restriction cohorts, respectively. The fat quantification correlation between FSE (with and without water suppression) sequences and Dixon for SAT and VAT were 0.9709, 0.9803 and 0.9955, 0.9840 respectively. The algorithm significantly reduced the computation time from 100 sec/slice to 25 sec/slice. The pre-processing, data-derived contour placement and avoidance of strong background-image boundary improved the convergence accuracy of the proposed algorithm. We developed a fully automatic segmentation algorithm to quantitate SAT and VAT from abdominal images of rodents, which can support large cohort studies. We additionally identified the influence of non-algorithmic variables including cradle disturbance, animal positioning, and MR sequence on the fat quantification. There were no large variations between FSE and Dixon-based estimation of SAT and VAT.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25310298</pmid><doi>10.1371/journal.pone.0108979</doi><oa>free_for_read</oa></addata></record>
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subjects Abdomen
Abdominal Fat - pathology
Adipose tissue
Algorithms
Animal tissues
Animals
Automation
Biology and Life Sciences
Body Fat Distribution
Body weight loss
Consortia
Data processing
Diabetes
Diet, High-Fat
Engineering and Technology
Evolutionary algorithms
Fuzzy logic
Gene expression
Health care
High fat diet
Image processing
Image segmentation
Insulin resistance
Laboratories
Magnetic resonance
Magnetic Resonance Imaging
Medical imaging
Medicine and Health Sciences
Metabolic disorders
Mice
NMR
Nuclear magnetic resonance
Nutrient deficiency
Obesity
Obesity - pathology
Rats
Reproducibility of Results
Rodents
Science
Spine
Vertebrae
Weight control
Weight Loss
title Quantification of abdominal fat depots in rats and mice during obesity and weight loss interventions
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