Real-time UV-visible spectroscopy analysis of purple membrane-polyacrylamide film formation taking into account Fano line shapes and scattering

We theoretically and experimentally analyze the formation of thick Purple Membrane (PM) polyacrylamide (PA) films by means of optical spectroscopy by considering the absorption of bacteriorhodopsin and scattering. We have applied semiclassical quantum mechanical techniques for the calculation of abs...

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Bibliographic Details
Published in:PloS one 2014-10, Vol.9 (10), p.e110518-e110518
Main Authors: Gomariz, María, Blaya, Salvador, Acebal, Pablo, Carretero, Luis
Format: Article
Language:English
Subjects:
Absorption
Absorption spectra
Acrylamide
Acrylic Resins - chemistry
Analysis
Bacteriorhodopsin
Bacteriorhodopsins - chemistry
Bioengineering
Biology
Biology and Life Sciences
Chemical synthesis
Chemistry
Engineering and Technology
Hydrogen-Ion Concentration
Kinetics
Light
Optical properties
Physical Sciences
Polyacrylamide
Polymer chemistry
Polymerization
Polymers
Protein Conformation
Proteins
Purple Membrane - chemistry
Quantum dots
Quantum mechanics
Rhodopsin
Scattering
Spectroscopy
Spectrum Analysis
Stem cells
Ultraviolet-visible spectroscopy
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Summary:We theoretically and experimentally analyze the formation of thick Purple Membrane (PM) polyacrylamide (PA) films by means of optical spectroscopy by considering the absorption of bacteriorhodopsin and scattering. We have applied semiclassical quantum mechanical techniques for the calculation of absorption spectra by taking into account the Fano effects on the ground state of bacteriorhodopsin. A model of the formation of PM-polyacrylamide films has been proposed based on the growth of polymeric chains around purple membrane. Experimentally, the temporal evolution of the polymerization process of acrylamide has been studied as function of the pH solution, obtaining a good correspondence to the proposed model. Thus, due to the formation of intermediate bacteriorhodopsin-doped nanogel, by controlling the polymerization process, an alternative methodology for the synthesis of bacteriorhodopsin-doped nanogels can be provided.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0110518