Identification of kernel proteins associated with the resistance to fusarium head blight in winter wheat (Triticum aestivum L.)

• Published in: PloS one 2014-10, Vol.9 (10), p.e110822-e110822
• Main Authors: Perlikowski, Dawid, Wiśniewska, Halina, Góral, Tomasz, Kwiatek, Michał, Majka, Maciej, Kosmala, Arkadiusz
• Format: Article
• Language: English
• Subjects: Abundance; alpha-Amylases - antagonists & inhibitors; alpha-Amylases - chemistry; Amylases; Biology and Life Sciences; Blight; Carbon - chemistry; Cereals; Crosses, Genetic; Cultivars; Disease Resistance - genetics; Electrophoresis, Gel, Two-Dimensional; Endosperm; Enzymatic activity; Enzyme activity; Enzymes; Festuca arundinacea; Fusarium; Fusarium - pathogenicity; Fusarium culmorum; Fusarium head blight; Gel electrophoresis; Gene expression; Genetics; Health aspects; Hydrolysis; Infections; Inoculation; Mass Spectrometry; Mass spectroscopy; Nitrogen - chemistry; Pathogens; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - physiology; Proteins; Proteome; Proteomics; Species Specificity; Triticosecale; Triticum - genetics; Triticum - microbiology; Triticum - physiology; Triticum aestivum; Wheat; Winter wheat; α-Amylase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0110822

Numerous potential components involved in the resistance to Fusarium head blight (FHB) in cereals have been indicated, however, our knowledge regarding this process is still limited and further work is required. Two winter wheat (Triticum aestivum L.) lines differing in their levels of resistance to FHB were analyzed to identify the most crucial proteins associated with resistance in this species. The presented work involved analysis of protein abundance in the kernel bulks of more resistant and more susceptible wheat lines using two-dimensional gel electrophoresis and mass spectrometry identification of proteins, which were differentially accumulated between the analyzed lines, after inoculation with F. culmorum under field conditions. All the obtained two-dimensional patterns were demonstrated to be well-resolved protein maps of kernel proteomes. Although, 11 proteins were shown to have significantly different abundance between these two groups of plants, only two are likely to be crucial and have a potential role in resistance to FHB. Monomeric alpha-amylase and dimeric alpha-amylase inhibitors, both highly accumulated in the more resistant line, after inoculation and in the control conditions. Fusarium pathogens can use hydrolytic enzymes, including amylases to colonize kernels and acquire nitrogen and carbon from the endosperm and we suggest that the inhibition of pathogen amylase activity could be one of the most crucial mechanisms to prevent infection progress in the analyzed wheat line with a higher resistance. Alpha-amylase activity assays confirmed this suggestion as it revealed the highest level of enzyme activity, after F. culmorum infection, in the line more susceptible to FHB.