Chikungunya virus glycoproteins pseudotype with lentiviral vectors and reveal a broad spectrum of cellular tropism

Outbreaks of the Chikungunya virus (CHIKV) infection has been documented in over 40 countries, resulting in clinical symptoms characterized by fever and joint pain. Diagnosing CHIKV in a clinical lab setting is often omitted because of the high lab safety requirement. An infection system that mimics...

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Bibliographic Details
Published in:PloS one 2014-10, Vol.9 (10), p.e110893-e110893
Main Authors: Hu, Dakang, Zhang, Jin, Wang, Hua, Liu, Shuangchun, Yu, Lianhua, Sun, Lingfen, Qu, Ying
Format: Article
Language:English
Subjects:
Aedes albopictus
Antibodies
Antibodies, Neutralizing - administration & dosage
Antibodies, Neutralizing - immunology
Antibodies, Viral - administration & dosage
Antibodies, Viral - immunology
Biology and Life Sciences
Biomedical laboratories
Biosafety
Cell lines
Chikungunya Fever - diagnosis
Chikungunya Fever - immunology
Chikungunya Fever - therapy
Chikungunya virus
Chikungunya virus - genetics
Chikungunya virus - immunology
Chikungunya virus - pathogenicity
Culicidae
Epidemics
Evaluation
Experiments
Fever
Genetic Vectors
Genomes
Glycoproteins
Glycoproteins - biosynthesis
Glycoproteins - genetics
Glycoproteins - immunology
Health aspects
Hospitals
Humans
Infection
Infections
Infectious diseases
Laboratories
Lentivirus
Luciferase
Medical treatment
Medicine
Mosquitoes
Mutation
Neutralizing
Outbreaks
Pain
Pathogens
Plasmids
Proteins
Structural proteins
Tropism
Vector-borne diseases
Viral genetics
Viral Structural Proteins - biosynthesis
Viral Structural Proteins - genetics
Viral Structural Proteins - immunology
Virology
Viruses
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Description
Summary:Outbreaks of the Chikungunya virus (CHIKV) infection has been documented in over 40 countries, resulting in clinical symptoms characterized by fever and joint pain. Diagnosing CHIKV in a clinical lab setting is often omitted because of the high lab safety requirement. An infection system that mimics CHIKV infection will permit clinical evaluation of the production of neutralizing antibody for both disease diagnostics and treatment. We generated a CHIKV construct expressing CHIKV structural proteins. This construct permits the production of CHIKV pseudo-viral particles with a luciferase reporter. The pseudo-virus was able to infect a wide range of cell lines. The pseudovirus could be neutralized by the addition of neutralizing antibodies from patients. Taken together, we have developed a powerful system that can be handled at biosafety level 2 laboratories for evaluation of existence of CHIKV neutralizing antibodies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0110893