Reduced heme levels underlie the exponential growth defect of the Shewanella oneidensis hfq mutant

The RNA chaperone Hfq fulfills important roles in small regulatory RNA (sRNA) function in many bacteria. Loss of Hfq in the dissimilatory metal reducing bacterium Shewanella oneidensis strain MR-1 results in slow exponential phase growth and a reduced terminal cell density at stationary phase. We ha...

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Bibliographic Details
Published in:PloS one 2014-10, Vol.9 (10), p.e109879-e109879
Main Authors: Brennan, Christopher M, Mazzucca, Nicholas Q, Mezoian, Taylor, Hunt, Taylor M, Keane, Meaghan L, Leonard, Jessica N, Scola, Shelby E, Beer, Emma N, Perdue, Sarah, Pellock, Brett J
Format: Article
Language:English
Subjects:
Aminolevulinic acid
Aminolevulinic Acid - metabolism
Bacteria
Bacteriology
Biochemistry
Biology
Biology and Life Sciences
Biosynthesis
Cell density
Chemical synthesis
Cytochrome
E coli
Enzymes
Escherichia coli
Gene expression
Genetic engineering
Genomes
Growth
Heme
Heme - biosynthesis
Heme - genetics
Hemoglobin synthesis
Host Factor 1 Protein - genetics
Host Factor 1 Protein - metabolism
Mutation
Oxidative stress
Physiological aspects
Physiology
Proteins
Ribonucleic acid
RNA
Salmonella
Salmonella Typhimurium
Shewanella - genetics
Shewanella - growth & development
Shewanella - metabolism
Stationary phase
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Summary:The RNA chaperone Hfq fulfills important roles in small regulatory RNA (sRNA) function in many bacteria. Loss of Hfq in the dissimilatory metal reducing bacterium Shewanella oneidensis strain MR-1 results in slow exponential phase growth and a reduced terminal cell density at stationary phase. We have found that the exponential phase growth defect of the hfq mutant in LB is the result of reduced heme levels. Both heme levels and exponential phase growth of the hfq mutant can be completely restored by supplementing LB medium with 5-aminolevulinic acid (5-ALA), the first committed intermediate synthesized during heme synthesis. Increasing expression of gtrA, which encodes the enzyme that catalyzes the first step in heme biosynthesis, also restores heme levels and exponential phase growth of the hfq mutant. Taken together, our data indicate that reduced heme levels are responsible for the exponential growth defect of the S. oneidensis hfq mutant in LB medium and suggest that the S. oneidensis hfq mutant is deficient in heme production at the 5-ALA synthesis step.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0109879