N-glycans: phenotypic homology and structural differences between myocardial cells and induced pluripotent stem cell-derived cardiomyocytes

• Published in: PloS one 2014-10, Vol.9 (10), p.e111064-e111064
• Main Authors: Kawamura, Takuji, Miyagawa, Shigeru, Fukushima, Satsuki, Yoshida, Akira, Kashiyama, Noriyuki, Kawamura, Ai, Ito, Emiko, Saito, Atsuhiro, Maeda, Akira, Eguchi, Hiroshi, Toda, Koichi, Lee, Jong-Kook, Miyagawa, Shuji, Sawa, Yoshiki
• Format: Article
• Language: English
• Subjects: Acids; Analysis; Animals; Biology and Life Sciences; Carbohydrate Conformation; Cardiomyocytes; Cell Differentiation; Cell Line; Cell surface; Chromatography; Differentiation; Elution; Glucose; Glycan; Heart; Heart cells; Heart surgery; High-performance liquid chromatography; Homology; Hydroxylase; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Kinases; Laboratory animals; Lasers; Liquid chromatography; Mannose; Medicine; Mice; Microscopy; Myocardium; Myocardium - metabolism; Myocytes, Cardiac - cytology; Myocytes, Cardiac - metabolism; N-Acetylneuraminic acid; N-glycans; Phenotypes; Pluripotency; Polysaccharides; Polysaccharides - metabolism; Proteomics; Stem cell transplantation; Stem cells; Transplants & implants; University graduates; Vectors (Biology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0111064

Cell surface glycans vary widely, depending on cell properties. We hypothesized that glycan expression on induced pluripotent stem cells (iPSCs) might change during cardiomyogenic differentiation toward the myocardial phenotype. N-glycans were isolated from iPSCs, iPSC-derived cardiomyocytes (iPSC-CM), and original C57BL/6 mouse myocardium (Heart). Their structures were analyzed by a mapping technique based on HPLC elution times and MALDI-TOF/MS spectra. Sixty-eight different N-glycans were isolated; the structures of 60 of these N-glycans were identified. The quantity of high-mannose type (immature) N-glycans on the iPSCs decreased with cardiomyogenic differentiation, but did not reach the low levels observed in the heart. We observed a similar reduction in neutral N-glycans and an increase in fucosylated or sialyl N-glycans. Some structural differences were detected between iPSC-CM and Heart. No N-glycolyl neuraminic acid (NeuGc) structures were detected in iPSC-CM, whereas the heart contained numerous NeuGc structures, corresponding to the expression of cytidine monophosphate-N-acetylneuraminic acid hydroxylase. Furthermore, several glycans containing Galα1-6 Gal, rarely identified in the other cells, were detected in the iPSC-CM. The expression of N-glycan on murine iPSCs changed toward the myocardial phenotype during cardiomyogenic differentiation, leaving the structural differences of NeuGc content or Galα1-6 Gal structures. Further studies will be warranted to reveal the meaning of the difference of N-glycans between the iPSC-CM and the myocardium.