Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability

• Published in: PloS one 2014-10, Vol.9 (10), p.e110834-e110834
• Main Authors: Yang, Jie, Lin, Qi, Ng, Tzi Bun, Ye, Xiuyun, Lin, Juan
• Format: Article
• Language: English
• Subjects: Basidiomycota - enzymology; Biodegradation; Biology and Life Sciences; Bioremediation; Calcium; Calcium ions; Carbohydrates; Chemical properties; Cloning; Coloring Agents - chemistry; Copper; Cultivation; Decoloring; Deoxyribonucleic acid; DNA; Dyes; Enzymes; Fermentation; Fungal Proteins - chemistry; Fungal Proteins - isolation & purification; Fungi; Gene expression; Gene sequencing; Iron; Laboratories; Laccase; Laccase - chemistry; Laccase - isolation & purification; Lignin; Magnesium; Metal ions; Organic solvents; Oxidases; Phylogenetics; Pleurotus ostreatus; Proteins; Purification; Regulatory sequences; Shaking; Substrates; Sulfonic acid; Textile Industry; Textile industry wastewaters; Transcription; Wastewater treatment; Water Purification; Zinc; Zinc compounds
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0110834

Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL(-1) was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg(-1) was purified. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was the optimal substrate, with Km and kcat being 93.4 µM and 2468.0 s(-1), respectively. LacA was stable at 60°C, pH 5.0 and above, and in organic solvents. Metal ions Na+, K+, Ca2+, Mg2+, Mn2+, Zn2+ enhanced LacA activity, while Fe2+ and Li+ inhibited LacA activity. LacA decolorized structurally different dyes and a real textile effluent. Its gene and cDNA sequences were obtained. Putative cis-acting transcriptional response elements were identified in the promoter region. The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp. HYB07 potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment.