Expression of the p53 target Wig-1 is associated with HPV status and patient survival in cervical carcinoma

• Published in: PloS one 2014-11, Vol.9 (11), p.e111125-e111125
• Main Authors: Xu, Li-Di, Muller, Susanne, Thoppe, Srinivasan R, Hellborg, Fredrik, Kanter, Lena, Lerner, Mikael, Zheng, Biying, Lagercrantz, Svetlana Bajalica, Grandér, Dan, Wallin, Keng Ling, Wiman, Klas G, Larsson, Catharina, Andersson, Sonia
• Format: Article
• Language: English
• Subjects: Apoptosis; Biology and Life Sciences; Biotechnology; Cancer; Carcinoma; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell cycle; Cell death; Cell Line, Tumor; Cell Nucleus - metabolism; Cervical cancer; Cervical carcinoma; Cervix; Children & youth; Childrens health; Chromosome 3; Chromosomes; Chromosomes, Human, Pair 3 - genetics; Comparative analysis; Cytogenetics; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genetic Loci - genetics; Gynecology; Hospitals; Human papillomavirus; Humans; Hybridization; Immunohistochemistry; Kinases; Lesions; Medical prognosis; Medicin och hälsovetenskap; Medicine and Health Sciences; Neoplasm Grading; Neoplasm Staging; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Obstetrics; Oncology; p53 Protein; Papillomaviridae - physiology; Papillomavirus infections; Pathology; Patient outcomes; Prognosis; Proteins; RNA; RNA-Binding Proteins; Senescence; Southern blotting; Staining; Survival; Survival Analysis; Telomerase; Tumor cell lines; Tumor proteins; Tumor Suppressor Protein p53 - metabolism; Tumors; Uterine Cervical Neoplasms - diagnosis; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; Womens health
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0111125

The p53 target gene WIG-1 (ZMAT3) is located in chromosomal region 3q26, that is frequently amplified in human tumors, including cervical cancer. We have examined the status of WIG-1 and the encoded Wig-1 protein in cervical carcinoma cell lines and tumor tissue samples. Our analysis of eight cervical cancer lines (Ca Ski, ME-180, MS751, SiHa, SW756, C-4I, C-33A, and HT-3) by spectral karyotype, comparative genomic hybridization and Southern blotting revealed WIG-1 is not the primary target for chromosome 3 gains. However, WIG-1/Wig-1 were readily expressed and WIG-1 mRNA expression was higher in the two HPV-negative cervical cell lines (C33-A, HT-3) than in HPV-positive lines. We then assessed Wig-1 expression by immunohistochemistry in 38 cervical tumor samples. We found higher nuclear Wig-1 expression levels in HPV-negative compared to HPV positive cases (p = 0.002) and in adenocarcinomas as compared to squamous cell lesions (p