Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site

TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3....

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-11, Vol.9 (11), p.e113096-e113096
Main Authors: Cocchiola, Rossana, Grillo, Caterina, Altieri, Fabio, Chichiarelli, Silvia, Turano, Carlo, Eufemi, Margherita
Format: Article
Language:English
Subjects:
Apoptosis
Binding sites
Binding Sites - genetics
Biochemistry, Molecular Biology
Biology and life sciences
Blotting, Western
Cancer
Cell Cycle
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Proliferation
Cells (Biology)
Chemical properties
Chromatin Immunoprecipitation
Chromosomes
Conserved sequence
Cytokines
Deoxyribonucleic acid
DNA
Electrophoretic Mobility Shift Assay
Experiments
Fluorescent Antibody Technique
Gene expression
Gene Expression Regulation, Neoplastic
Genes
Genetic engineering
Growth factors
Humans
Kinases
Life Sciences
Medicine and Health Sciences
Melanoma
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Mitosis
Neoplasms - genetics
Neoplasms - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Overexpression
Promoter Regions, Genetic
Protein binding
Proteins
Real-Time Polymerase Chain Reaction
Regulatory Sequences, Nucleic Acid
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Stat3 protein
STAT3 Transcription Factor - genetics
STAT3 Transcription Factor - metabolism
Thyroid gland
Tissues
Trans-Activators - metabolism
Transcription factors
Transcriptional Activation
Tumor Cells, Cultured
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0113096