Rapidly-deposited polydopamine coating via high temperature and vigorous stirring: formation, characterization and biofunctional evaluation

• Published in: PloS one 2014-11, Vol.9 (11), p.e113087-e113087
• Main Authors: Zhou, Ping, Deng, Yi, Lyu, Beier, Zhang, Ranran, Zhang, Hai, Ma, Hongwei, Lyu, Yalin, Wei, Shicheng
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacterial Adhesion - drug effects; Biological products; Biology and Life Sciences; Biomaterials; Biomedical materials; Biomolecules; Biosensing Techniques - methods; Cattle; Cell Line, Tumor; Cell Survival - drug effects; Coating; Coatings; Dentistry; Deposition; Dopamine; Engineering and Technology; Escherichia coli - physiology; High temperature; Hot Temperature; Humans; Immobilization; Indoles - chemistry; Indoles - pharmacology; Interdisciplinary aspects; Kinetics; Maxillofacial surgery; Medical devices; Medical equipment; Membranes, Artificial; Microscopy, Atomic Force; Photoelectron Spectroscopy; Physical Sciences; Polymerization; Polymers; Polymers - chemistry; Polymers - pharmacology; Quartz crystal microbalance; Quartz Crystal Microbalance Techniques - methods; Reaction kinetics; Sensors; Serum Albumin, Bovine - chemistry; Silicon wafers; Spectroscopy, Fourier Transform Infrared; Stirring; Streptococcus mutans - physiology; Studies; Substrates; Surface Properties; Surgery; Temperature effects; Time Factors; Tissue engineering; Ultrasonics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0113087

Polydopamine (PDA) coating provides a promising approach for immobilization of biomolecules onto almost all kinds of solid substrates. However, the deposition kinetics of PDA coating as a function of temperature and reaction method is not well elucidated. Since dopamine self-polymerization usually takes a long time, therefore, rapid-formation of PDA film becomes imperative for surface modification of biomaterials and medical devices. In the present study, a practical method for preparation of rapidly-deposited PDA coating was developed using a uniquely designed device, and the kinetics of dopamine self-polymerization was investigated by QCM sensor system. It was found that high temperature and vigorous stirring could dramatically speed up the formation of PDA film on QCM chip surface. Surface characterization, BSA binding study, cell viability assay and antibacterial test demonstrates that the polydopamine coating after polymerization for 30 min by our approach exhibits similar properties to those of 24 h counterpart. The method has a great potential for rapid-deposition of polydopamine films to modify biomaterial surfaces.