Electronically monitored labial dabbing and stylet 'probing' behaviors of brown marmorated stink bug, Halyomorpha halys, in simulated environments

• Published in: PloS one 2014-12, Vol.9 (12), p.e113514
• Main Authors: Wiman, Nik G, Walton, Vaughn M, Shearer, Peter W, Rondon, Silvia I
• Format: Article
• Language: English
• Subjects: Agricultural economics; Agriculture; Animal behavior; Animals; Beans; Biology and Life Sciences; Economic importance; Engineering and Technology; Environment; Environmental chambers; Environmental conditions; Feeding; Feeding Behavior; Hemiptera - physiology; Insects; Invasive insects; Nighttime; Periodicity; Pests; Probes; Proboscis; Seasonal variations; Stink bugs; Temperature; Temperature effects; Test chambers; Urban agriculture
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0113514

Brown marmorated stink bug, Halyomorpha halys (Stål), (Hemiptera: Pentatomidae) is an invasive polyphagous agricultural and urban nuisance pest of Asian origin that is becoming widespread in North America and Europe. Despite the economic importance of pentatomid pests worldwide, their feeding behavior is poorly understood. Electronically monitored insect feeding (EMIF) technology is a useful tool in studies of feeding behavior of Hemiptera. Here we examined H. halys feeding behavior using an EMIF system designed for high throughput studies in environmental chambers. Our objectives were to quantify feeding activity by monitoring proboscis contacts with green beans, including labial dabbing and stylet penetration of the beans, which we collectively define as 'probes'. We examined frequency and duration of 'probes' in field-collected H. halys over 48 hours and we determined how environmental conditions could affect diel and seasonal periodicity of 'probing' activity. We found differences in 'probing' activity between months when the assays were conducted. These differences in activity may have reflected different environmental conditions, and they also coincide with what is known about the phenology of H. halys. While a substantial number of 'probes' occurred during scotophase, including some of the longest mean 'probe' durations, activity was either lower or similar to 'probing' activity levels during photophase on average. We found that temperature had a significant impact on H. halys 'probing' behavior and may influence periodicity of activity. Our data suggest that the minimal temperature at which 'probing' of H. halys occurs is between 3.5 and 6.1 °C (95% CI), and that 'probing' does not occur at temperatures above 26.5 to 29.6 °C (95% CI). We estimated that the optimal temperature for 'probing' is between 16 and 17 °C.